组织工程化类金刚石膜复合材料与人血管内皮细胞相容性的研究

Tissue engineering diamond-like carbon (DLC) film complex material with human blood vessel endothelial cell biocompatibility nature research

目的:利用脉冲激光沉积(PLD)方法在人工心脏机械瓣膜上沉积纳米相类金刚石(DLC)薄膜,探讨纳米相类金刚石薄膜复合材料与人血管内皮细胞相容性,为应用组织工程方法构建组织工程化机械瓣膜材料提供依据。方法:将人血管内皮细胞与纳米相类金刚石薄膜复合材料体外复合培养,进行形态学和功能测定,同时分别测定在DLC材料组和空白对照组中血管内皮细胞分泌一氧化氮(NO)、前列环素(PGI2)水平。结果:人血管内皮细胞能在纳米相类金刚石薄膜复合材料上良好地黏附、增殖、生长。人脐静脉血管内皮细胞分泌一氧化氮(NO)和前列环素(PGI2)在DLC材料和空白对照组差异无统计学意义(P>0.05)。结论:纳米相类金刚石薄膜复合材料具有良好的细胞相容性,可作为组织工程化机械瓣膜材料。

Objective：This item make use of impulse laser sediment（PLD） method at manual work heart instrument of valve sediment diamond-like carbon（DLC） film, Discuss DLC film complex material and human blood the vascnlar endothelial cell biocompatibility nature in vitro, in order to apply the tissue engineering method to construct tissue engineering manual work heart instrument valve material and provide thereunder. Method：Human the vascular endothelial cell with DLC film complex material complex cultivate, carry through morphological function mensurate, Simultaneity separate Analysis the secretory levels of nitric oxide （NO） and PGI2 by mensurate at DLC material group and blank comparison group human the vascular endothelial cell secrete NO and PGI2 level. Result： Human the vascular endothelial cell can adhere to multiplication, growth, human the vascular endothelial cell secrete NO and PGI2 level at DLC material group and blank comparison group without notability nature difference （P 〉 0.05）. Show DLC film complex material to human the vascular endothelial cell without disadvantage, and DLC film complex material to human the vascular endothelial cell without alive influence. Conelusion：DLC film complex material have very cell biocompatibility nature, can become tissue engineering manual work heart instrument valve material.