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消癥煎膏直肠给药诱导大鼠子宫肌瘤细胞凋亡的研究 被引量:1

Study on Hysteromyoma Apoptosis Induced by Mass-Clearing Extraction per Rectum
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摘要 目的:观察直肠给药(消癥煎膏)对子宫肌瘤模型鼠肌瘤细胞凋亡的干预作用。方法:SD雌性大鼠(正常动物对照组除外)参照FujiiS法,造子宫肌瘤模型,随机分成5组,即动物模型组、消癥煎膏高、中、低剂量组、桂枝茯苓胶囊对照组。放射免疫法测定雌二醇水平,免疫组化法观察病理形态学的变化以及细胞凋亡基因Bcl-2蛋白的表达。结果:高剂量组消癥煎膏能显著抑制模型大鼠瘤样增生(P<0.01),并降低模型大鼠血液中雌激素的水平(P<0.01)。模型组平滑肌Bcl-2蛋白阳性点数量明显增多,阳性信号显著增强,Bcl-2表达明显增多(P<0.01)。结论:消癥煎膏具有诱导子宫肌瘤细胞凋亡的作用,能降低子宫肌瘤模型鼠细胞凋亡蛋白Bcl-2阳性表达,抑制子宫肌瘤细胞增殖,缩小子宫肌瘤。 Objective:To observe the intervention of Mass-Clearing Extraction (per rectum) on hysteromyoma apoptosis of rat model. Methods:hysteromyoma models were established on SD female rats according to Fujii S methods, and randomly divided to 5 groups, ie. The model group, high-dosage, middle-level dosage,low-dosage group of the extraction, the control group (administered on Guizhi Fuling Jiaonang,Cinnamon Twig and Poria Capsule ). Estradiol levels were detected by radio-immunity method; pathomorphological changes and expression of Bcl-2, a gene of apoptosis were observed by immune histochemistry methods. Results : high dosage of the extraction can inhibit tumor related acerementition ( P 〈 0. 01 ) , and bring down cstrin level ( P 〈 0. 01 ). In the model group there were increased counting of positive cell of Bcl-2, intensified positive signal and more expression of Bel-2 ( P 〈 0.01 ). Conclusion: Mass-Clearing Extraction can diminish hysteromyoma by inducing apoptosis of hysteromyoma, reducing Bcl-2 positive expression and inhibit hysteromyoma accrementition.
出处 《世界中医药》 CAS 2008年第6期372-374,共3页 World Chinese Medicine
关键词 子宫肌瘤 中医药疗法 消癥煎膏 临床研究 Hysteromyoma/ Chinese Medical Therapy @ Mass-Clearing Extraction
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