乌司他丁对心肺复苏后大鼠脑组织含水量及ICAM-1的影响

目的探讨乌司他丁对心肺复苏早期大鼠脑组织含水量及脑组织ICAM-1水平的影响。方法将30只SD大鼠随机分为假手术对照组、心肺复苏(CPR)组、乌司他丁组,每组各10只大鼠。于自主呼吸循环恢复后2h,采用干湿法检测各组大鼠脑组织含水量,采用ELISE法检测大鼠脑组织ICAM-1水平。结果①脑组织含水量:CPR组为(80.4±2.0)%,较对照组(76.7±1.3)%显著增加(P<0.01);乌司他丁组(77.6±1.5)%,较CPR组显著减少(P<0.01)。②脑组织ICAM-1含量:CPR组为(207.448±37.279)ng/mg,较对照组(113.447±31.116)ng/mg明显增高(P<0.05);乌司他丁组(182.222±74.371)ng/mg,较CPR组显著减少(P<0.05)。结论CPR早期即可发生脑水肿,脑组织ICAM-1水平显著增加;应用乌司他丁能有效降低脑组织含水量和脑组织ICAM-1水平。

Objective To observe the effects of UTI on the moisture capacity and ICAM-1 of brains in CPR rats. Methods 30 grown male Sprague Dawley （SD） rats were divided randomly into there groups： control group, resuscitation group, UTI group. Each group had 10 rats. The last two groups were used to make CPR models induced by asphyxia and were observed lasting 2 hours after ROSC. Then all rats were caused dead and brain tissues were took out. Brain tissues were weighed （humid weight）, then they were dried in a baker and scaled dry weight. Moisture capacities were calculated by Elliott method. The lever of ICAM-1 in brain tissue was determined by ELISA method. Results ① The brain water content in the resuscitation group was significantly higher than that in the control group（80.4 ± 2.0% vs 76.7± 1.3%, P〈0.01）;The brain water content in UTI group was decreased significantly than that in the resuscitation group （77.6± 1.5% vs 80.4 ±2.0% ,P〈0.01）. ② The levers of ICAM-1 of brain tissues in the resuscitation group increased significantly than those in the control group （207.448 ±37.279ng/mg vs 113.44±31.116ng/mg,P〈0.05）;The levers of ICAM-1 of brain tissues in UTI group decreased significantly than those in the resuscitation group （182.222 ± 74.371ng/mg vs 207.448 ±37.279ng/mg,P〈0.05）. Conclusion Brain edema occurs during the early stage of CPR,and the levers of ICAM-1 in brain tissues increase significantly. The use of UTI may effectively reduce the brain water content, the levers of ICAM-1 in brain tissues.