摘要
目的为了探讨血清胰岛素(INS)和C肽(C-P)释放试验放射免疫分析法(RIA)与时间分辨免疫荧光分析法(TRIFA)检测结果的差异及各自优势。方法采用RIA法和TRFIA法对30例糖尿病患者的空腹﹑餐后30分钟﹑60分钟﹑120分钟血清INS和血清C-P水平进行检测。结果糖尿病组血清胰岛素空腹﹑餐后30分钟﹑60分钟﹑120分钟TRFIA法测定值显著低于RIA法测定值(p〈0.01);血清C肽空腹﹑餐后30分钟﹑60分钟﹑120分钟TRFIA法测定值显著高于RIA法测定值(p〈0.01)。结论RIA法与TRFIA法检测结果具有显著性差异,表明两种方法的INS﹑C-P的测定结果不能混用。
Objective To determine the difference and advantage of Serum Insulin （INS）and C-P by radioimmunoassay （RIA）and Time-resolved Fluorescence Immunoassay （TRFIA）. Methods The concentrations of serum INS and C-P at fasting,postprandial 30m, postprandial 60m and postprandial 120m was detected by RIA and TRFIA in 30 diabetic patients. Results The determinations of serum INS and C-P at fasting,postprandial 30m ,postprandial 60m and postprandial 120m by TRFIA was lower than the one by RIA（P〈0.01 ）. Conclusion The determinations was significant differences by RIA and TRFIA, that the results of the two methods can not mix.
