三叶青黄酮对H-22荷瘤小鼠瘤体的抑制作用及其机理研究

Inhibition Effect of Tetrastigma Hemsleyanum Diels et Gilg Flavone on H-22 Solid Tumors in Mice

[目的]观察三叶青总黄酮对H-22荷瘤小鼠瘤体的抑制作用,探讨其作用机理。[方法]小鼠肝癌H-22细胞右前肢腋窝皮下接种造膜,观察低、中、高剂量三叶青黄酮对荷瘤小鼠抑瘤率、脾脏指数、胸腺指数的影响,并通过定量RT-PCR检测小鼠皮下移植瘤Fasp-3、CytC的表达情况。[结果]低、中、高剂量三叶青黄酮对H-22荷瘤小鼠的瘤重抑制率分别为34.28%、32.56%和29.24%;皮下移植瘤Fasp-3、CytC mRNA的表达低剂量组明显高于空白组(P<0.05)。[结论]三叶青黄酮可显著抑制肿瘤细胞,这一作用与其能促进肿瘤细胞的促凋亡基因Fasp-3、CytC的表达相关。

[Objective]Observe H-22 solid tumor growth inhibition by Tetrastigma hemsleyanum Diels et Gilg flavone（TDGF） and analyse its mechanism. [Methods]Solid tumor-bearing Kunming mice were obtained using mice hepatoma H-22 cell line, tumor growth inhibition ratio of TDGF was assessed and compared, thymus index and spleen index were also recorded to evaluate the function of TDGF; Moreover,the gene expression level of Fasp-3,CytC were detected through quantitate RT-PCR. [Results]Low, moderate and high dose treatment groups showed inhibition H-22 solid tumor activity with inhibition ratio 34.28%, 32.56% and 29.24% respectively. Gene analysis showed that low dose group can up-regulate Fasp-3,CytC mRNA expression with significant deviation compared with control group（P〈0.05）. [Conclusion]TDGF can inhibit H-22 solid tumor growth in vivo, which maybe educed by up-regulating the expression of apoptosis related genes Fast〉3 and CytC.