白藜芦醇对淋巴瘤细胞增殖及细胞因子分泌的影响

The Effects of Res on Expression and Secretion of IL-8 and VEGF of Lymphoma Raji Cells

[目的]探讨白藜芦醇(resveratrol,Res)对淋巴瘤Raji细胞增殖及细胞因子白介素-8(IL-8)和血管内皮细胞生成因子(VEGF)基因表达和分泌的影响。[方法]用不同浓度白藜芦醇处理Raji细胞48h后,用MTT法检测细胞增殖指数,用ELISA法检测IL-8和VEGF蛋白含量,用逆转录/聚合酶链反应(RT/PCR)检测IL-8和VEGF mRNA表达情况。[结果]白藜芦醇体外对Raji细胞无增殖抑制作用,不同浓度白藜芦醇作用Raji细胞48h后,白藜芦醇1组、2组和3组IL-8、细胞上清液VEGF蛋白含量,均显著低于对照组(P<0.05);IL-8、VEGF mRNA表达相对强度,均显著低于对照组(P<0.05),且随浓度增加而减弱。[结论]白藜芦醇体外对Raji细胞无增殖抑制作用,但可抑制IL-8和VEGF的分泌及mRNA的表达,这可能是白藜芦醇抗肿瘤血管生成的重要机制。

[Objective] We aimed to investigate the effects of angiogenic inhibitor vitro. [Method] We examined the serum contents of IL-8 and VEGF in supernatant, by specific ELISAs of Raji cells after treatment by Res of different dosage（25umol/1-100umol/l）. The expression of IL-8 and VEGF mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR）. [Results] The protein contents of IL-8 in supernatant of Res（25umol/1-100umol/l） were（532.3 4- 54.6）pg/ml, （487.3± 63.0） pg/ml and （380.9 ±47.9） pg/ml respectively, significantly lower than that in the control. The protein contents of VEGF in supernatant of Res （25umol/1-100umol/l） were （73.3 ± 10.9） pg/ml, （46.6 ± 19.8） pg/mt, （30.2 ± 12.8） pg/ml respectively, significantly lower than that in the control. The relative values of expression of IL-8 mRNA in treated groups （25umol/1-100umol/l） were （0. 21± 0.06 ）, （0. 17±0.09） and （0. 12±0.07） respectively. The values of expression of IL-8 mRNA were significantly lower than control group. And the values were in inverse correlation with concentration. The relative values of expression of VEGF mRNA in treated groups were （0.46± 0.21）, （ 0.21 ± 0.13） and （ 0.09± 0.08） respectively. The values of expression of VEGF mRNA were significantly lower than control group and were in negative correlation with concentration. [Conclusions] These findings suggest that Res may inhibt the expression and secretion of IL-8 and VEGF, which may be one of the most important mechanisms.