摘要
[目的]建立HPLC法测定抱龙丸中厚朴酚及和厚朴酚的含量的方法。[方法]采用Agilent C18(4.6mm×250mm,5μm)色谱柱;流动相为甲醇-水(78∶22);流速1.0ml/min;检测波长294nm。[结果]厚朴酚的线性范围为0.02-0.26μg,r=0.9999,平均回收率为100.46%,RSD=1.00%;和厚朴酚线性范围为0.012-0.156μg,r=1,平均回收率为100.76%,RSD=1.37%。[结论]该方法结果准确,重现性好,可作为抱龙丸质量控制的定量方法。
[Objective] To establish an HPLC method for the determination of magnolol and honokiol in Baolong Pill. [Methods] The samples were analyzed on an Agilent C18 column（4.6mm× 250mm,5um）. The mobile phase was a mixture of MeOH--H2O （78 ： 22）;the rate of flow was 1.0 ml/min;the detection wave-length was 294 nm. [Results]Calibration graph was linear in the range of 0. 02- 0.26ug, r = 0. 999 9 for magnolol; 0. 012 - 0. 156ug, r = 1 for honokiol. The recovery was 100.46%, RSD= 1.000% for magnolol;100.76 %,RSD= 1.37% for honokiol. [Conclusion] The method is sensitive and its results are accurate with good reproducibility. It can be used for the quality control of Baolong Wan.
出处
《浙江中医药大学学报》
CAS
2008年第6期808-809,811,共3页
Journal of Zhejiang Chinese Medical University
