耐亚胺培南铜绿假单胞菌金属β-内酰胺酶基因研究

Drug Resistance Genes in Imipenem-resistant Pseudomonas aeruginosa

目的对耐亚胺培南铜绿假单胞菌进行相关耐药基因研究。方法采用聚合酶链反应(PCR)法对耐亚胺培南铜绿假单胞菌相关的金属β-内酰胺酶IMP、VIM、SPM、GIM基因进行检测与分析。结果62株耐亚胺培南铜绿假单胞菌中16株IMP和5株VIM型金属酶基因阳性,SPM和GIM型金属酶基因全部为阴性。结论铜绿假单胞菌产金属β-内酰胺酶是泰安地区该菌对亚胺培南耐药的重要机制。

OBJECTIVE To study the drug resistance genes in imipenem-resistant Pseudomonas aeruginosa. METHODS Five main drug resistance genes.. IMP, VIM, SPM and GIM which are pertinent with metal β- lactamase and outer membrane protein oprD2 gene were analyzed using PCR method. RESULTS The oprD2 genes in 48 strains of imipenem-resistant P. aeruginosa were negative, and in 14 strains were positive from all 62 strains tested. There were 16 strains with positive IMP type and 5 strains with positive VIM type metal enzyme genes positive, 7 metal enzymes producing strains were with negative oprD2 gene. The SPM and GIM metal enzyme genes detected were all negative. CONCLUSIONS The loss of Outer membrane protein oprD2 gene is the main mechanism of imipenem resistance in P. aeruginosa in Tai＇an area.