抗梅毒螺旋体主要抗体与RPR的关系探讨

Relationship Between Major Antibodies of Treponema pallidum and RPR

目的分析梅毒阳性血清抗TP15、17、47单组分抗体的检出情况并探讨其与梅毒心磷脂反应素的关系。方法分别建立抗TP15、174、7 ELISA双抗体夹心法检测梅毒阳性血清,再用苍白密螺旋体颗粒(明胶)凝集试验(TPPA)法确定,同时快速血浆反应素试验(RPR)法测定抗心磷脂反应素阳性滴定度。结果抗TP15、17、47与常规ELISA的阳性不符合率分别为15.2%,3.3%和5.1%,各组分抗体强度的分布不同;ELISA检测的OD值与TPPA检测时血清的抗体强度无相关性(r=0.073);比较3种抗TP单组分抗体的OD值在RPR阳性和阴性组的差异均有统计学意义。结论抗TP15、17、47单组分抗体与ELISA有不同的阳性不符合率,ELISA与TPPA检测的血清抗体强度无相关性;RPR的阳性检出率为47.1%,3种抗TP单组分抗体均为阳性而RPR阳性组OD值低于阴性组。

OBJECTIVE To analyze the results of Treponema pallidum positive serum single antibody including three antibodies （anti-TP15, anti-TP17, and anti-TP47）, and to discuss the relationship between the results and cardiolipin （diphosphatidyl glycerol） reagin. METHODS Detecting T. pallidum positive serum by enzyme-linked immunosorbent assay （ELISA） with established anti-TP15, anti-TP17, and anti-TP47, respectively, and then, confirmed by TPPA, the titer of anticardiolipin reagin was measured by rapid plasma reagin test （RPR）, simultaneously. RESULTS The disagreement between three autibodies and ELISA was 15.2 %, 3.3 % and 5.1%, respectively, and distribution of their antibody intensity was different. There was no correlation between the value of OD of ELISA and antibody intensity in T. pallidum particle agglutination test （TPPA）. The difference was significant in comparison to the value of OD of these anti-TPs between negative and positive RPR. CONCLUSIONS The disagreement is not the same between these three antibodies and ELISA. There is no correlation between the value of OD of ELISA and antibody intensity in TPPA. The detection rate of RPR is 47.1 %, The value of OD in RPR positive group is lower than that in RPR negative group when these three antibodies are positive.