多位点序列分型及其应用

Multilocus sequence typing and its application

多位点序列分型(multilocus sequence typing,MLST)是一种基于核酸序列测定的细菌分型方法。这种方法通过PCR扩增多个管家基因内部片段并测定其序列,分析菌株的变异。MLST操作简单,结果能快速得到并且便于不同实验室的比较,已经用于多种细菌的流行病学监测和进化研究。随着测序速度的加快和成本的降低,以及分析软件的发展,MLST逐渐成为细菌的常规分型方法。

Multilocus sequence typing （MLST） was proposed as a nucleotide sequence-based approach that could be applied to many bacterial pathogens. It analyzed allelic variation at multiple housekeeping loci by nucleotide sequencing of internal fragments. MLST combined developments in high-throughput sequencing and bioinformatics with established population genetics techniques to provide a portable, reproducible, and scalable typing system that reflected the population and evolutionary biology of bacterial pathogens.