摘要
目的观察致敏的树突细胞(DC)治疗膀胱肿瘤(BT)后血液细胞毒性T淋巴细胞(CTL)的变化并探讨其机制。方法选取F344大鼠44只,称重后随机分为4组;均采用膀胱灌注N-甲基亚硝基脲(MNU)制成BT;第11周4组分别经皮下注射磷酸缓冲液(PBS)、未致敏DC、肿瘤抗原及致敏DC,每周1次,共4次;实验第15周,经显微镜和流式细胞仪(FCM)检测。结果致敏DC组膀胱重量比其他三组轻(P〈0.05)。致敏DC组BT病理分期低于PBS组和未致敏DC组(P〈0.05);CD3^+ T细胞在致敏DC组低于其余三组(P〈0.05);CTL在致敏DC组高于其余三组(P〈0.001)。结论应用致敏DC经皮下注射治疗大鼠BT可降低肿瘤的病理分期,未致敏DC皮下注射对膀胱肿瘤治疗无效,肿瘤抗原皮下注射对膀胱肿瘤治疗效果欠佳。致敏DC可通过呈递抗原来激活CTL增生而发挥其免疫杀伤作用。
Objective To study the effects of sensitized dendritic cells in the treatment of bladder tumor and further discuss the mechanism of this immunotherapy. Methods 44 female F344 rats, which irrigated N-methyl-N-nitrosourea into bladders every other week for a total of five doses, were induced to bladder tumor. They were treated subcutaneously with either PBS, unsensitized DC, freeze thawing supernatant of tumor cells, or sensitized DC respectively every week for a total of four times. In the fifteenth week, their bladders were weighted and harvested for observation by naked eye and microscope, their blood was harvested for examination CTL by FCM. Results The weight of bladders in sensitized DC group was lower than those in PBS group, unsensitized DC group and freeze thawing supernatant of bladder tumor cells group (P 〈0.05). The stages of bladder tumor in sensitized DC group were statistically descended compared with those in PBS group (P 〈0.05). The CD3^+ T cells in sensitized DC group was lower than those in PBS group, unsensitized DC group and freeze thawing supernatant of bladder tumor cells group (P 〈0.05). The CD; CD8^+ CD28^+ T cells in sensitized DC group was higher than those in PBS group, unsensitized DC group and freeze thawing supematant of bladder tumor cells group(P 〈0.001). Conclusion Sensitized DC injecting subcutaneously can reduce the stages of F344 rats' bladder tumor. Unsensitized DC injecting subcutaneously has not effect in the treatment of bladder tumor; while the effect of freeze thawing supernatant of tumor cells injecting subcutaneously is not well. The mechanism of sensitized DC in the treatment of blader tumor is that DC plays an immunol killing role by presenting antigen, stimulating CTL.
出处
《肿瘤研究与临床》
CAS
2008年第11期737-739,共3页
Cancer Research and Clinic
基金
山西省自然科学基金(20051097)
山西省回国留学人员科研资助项目(02082)