血吸虫病患者肝癌细胞p53和c-myc蛋白的表达

Expression and significance of p53 and c-myc in hepatocellular carcinoma with schistosomiasis

目的对血吸虫病患者肝癌细胞p53和c-myc蛋白的表达进行研究,探讨血吸虫感染与肝细胞癌的关系。方法将58例原发性肝细胞癌患者分为两组:原发性肝细胞癌伴有血吸虫病组(HCS组)23例和原发性肝细胞癌不伴有血吸虫病组(HC组)35例。采用免疫组织化学S-P法检测所有患者的p53蛋白和c-myc蛋白的表达,同时对p53、c-myc与肝细胞癌的多种临床病理参数的相关性进行分析。结果p53蛋白阳性表达率在HCS组和HC组中分别为73.9%和31.4%,两组间比较有显著性差异(P<0.01);p53及c-myc在肝癌组织中的检出率与淋巴结转移、术后复发及肿瘤分化程度明显相关(P<0.05或0.01)。结论血吸虫感染对肝癌细胞中p53突变蛋白的过量表达有一定促进作用;p53及c-myc在肝癌组织中的检出率与代表肿瘤不良生物学特征的指标淋巴结转移、术后复发及肿瘤分化程度明显相关。

Objective To evaluate the correlation between the expression of p53 and c- myc genes and the Obiological behavior of hepatocellular carcinoma with schistosomiasis. Methods 58 patients of hepatocellular carcinoma were selected and divided into two groups：group HCS （hepatocellular carcino- ma with schistosomiasis, n = 23 ）and group HC （hepatocellular carcinoma without schistosomiasis, n = 35 ）. Immunohistochemistry method were used in all patients to detect the p53 and c-myc proteins expression. Meanwhile, the relationship of the expression of p53 and c-myc with several chnicopathologic factors such as extrahepatic metastasis, the recurrence of tumor and the differentiation of tumor was analyzed. Results ①The positive rate of p53 protein expression in group HCS and group HC were 73.9% and 31.4% respectively,and there were significant differences between two groups（P 〈 0.01 ）.②The positive rates of p53 and c-myc in the HCC tissue were significantly correlated with the presence of extrahepatic metastasis ,the recurrence of tumor and the differentiation of tumor（ P 〈 0.05 or 0.01 ）. Conclusion There is higher gene mutation rate in the patients of hepatocellular carcinoma complicated with schistosomiasis, which suggests that schistosomiasis can promote p53 mutation protein over-expression in the histiocytes of HCC. The over-expression of c-myc and p53 proteins may contribute to the proliferation of hepatoma cell. The positive rates of p53 and c-myc in the HCC tissue were significantly correlated with the presence of extrahepatic metastasis,the recurrence of tumor and the differentiation of tumor.