摘要
目的初步观察外源性细胞因子重组人促红细胞生成素(rhEPO)对小鼠胚胎干细胞(ESC)向心肌细胞分化的影响。方法将rhEPO加到细胞培养基中配成终浓度分别为0.5、1.0、5.0U/ml的诱导分化液,分别在分化第8、12天观察ESC自发及在rhEPO诱导情况下心肌细胞分化率;在分化第7天应用RT-PCR检测自发分化组及rhEPO诱导组心肌早期转录因子GATA-4、Nkx2.5mRNA的相对表达。结果在分化第8、12天rhEPO诱导(1.0U/ml)心肌细胞分化率分别显著高于对应分化天数的自发心肌细胞分化率(0.11±0.02对0.05±0.01,P<0.05;0.86±0.02对0.65±0.05,P<0.05);与自发分化组比较,rhEPO干预(1.0U/ml)上调GATA-4、Nkx2.5mRNA的相对表达。结论外源性rhEPO早期干预能够促进ESC向心肌细胞分化。
Objective To study the effect of recombinant human erythropoietin （rhEPO） on cardiomyocytes （CM） differentiation from embryonic stem cells （ESC） in mice. Methods Compared the differentiation rate of CM at day 8 and 12 of differentiation in mouse embryo between groups with or without rhEPO treatment at indicated concentration （0.5,1.0,5.0 U/ml）.The expression of cardiac-restricted transcription factors Nkx2.5, GATA-4 at mRNA levels were measured with reverse transcription-polymerase chain reaction （RT-PCR） at day 7 of differentiation in mouse embryo. Results Compared to group of spontaneously differentiation ,the differentiation rate of CM at day 8 and 12 of differentiation in mouse embryo were significantly higher in the presence of rhEPO respectively （0.11 ± 0.02 vs 0.05± 0.01, P 〈 0.05 ;0.86 ± 0.02 vs 0.65±0.05,P 〈 0.05） ;rhEPO at 1.0 U/ml significantly up-regulated the mRNA levels of Nkx2.5 and GATA-4 at early stage of differentiation. Conclusion It is demonstrated that exogenous rhEPO promotes differentiation of CM from routine ESC.
出处
《生物医学工程与临床》
CAS
2008年第6期499-502,共4页
Biomedical Engineering and Clinical Medicine
基金
上海市科委重大项目子课题(03DJ14019)
