MMP-2、MMP-9在肺癌中的表达及其与肺癌转移和预后关系的研究

A Study on the Relationship Between Expression of MMP-2,MMP-9 and Metastasis and Prognosis in Patients with Lung Cancer

目的:探讨非小细胞肺癌(NSCLC)组织中基质金属蛋白酶-2(MMP-2)及基质金属蛋白酶-9(MMP-9)的表达水平与肺癌转移和预后的关系。方法:应用免疫组化(LSAB法)检测了108例非小细胞肺癌和19例肺良性病变中MMP-2、MMP-9的表达水平。结果:(1)肺癌组织中MMP-2、MMP-9的表达水平均显著高于癌旁肺组织和肺良性病变肺组织(P<0.01)。(2)伴有淋巴结或/和远外转移的肺癌中MMP-2、MMP-9的表达水平均显著高于不伴有淋巴结或/和远外转移的肺癌(P<0.01或P<0.05)。(3)MMP-2、MMP-9在肺鳞癌中的表达水平显著高于在腺癌和腺鳞癌中的表达水平(P<0.01)。(4)肺癌组织中MMP-2、MMP-9的表达水平呈显著正相关(P<0.05)。(5)多因素COX比例风险模型分析显示:MMP-2、MMP-9的表达水平对于预测肺癌预后具有一定意义。结论:(1)肺癌中MMP-2、MMP-9的表达水平明显升高,且与肺癌的进展和转移有密切关系。(2)肺癌组织中MMP-2、MMP-9的表达水平呈显著正相关(P<0.05)。(3)检测肺癌中MMP-2、MMP-9的表达水平有助于预测肺癌的预后,指导肺癌的多学科综合治疗。

Objective：To study the relationship between expression of MMP-2 MMP-9 and metastasis and prognosis in patients with lung cancer.Methods：The expression of MMP-2 and MMP-9 were detected in 108 non-small cell lung cancer tissues and 19 benign pulmonary lesion tissues by immunohistochemistry method（LSAB method）.Results：（1）the expression of MMP-2,MMP-9 in lung cancer tissues was significantly higher than that in adjacent non-cancerous lung tissues and benign pulmonary lesion（P〈0.05）.（2）Compared to the cases without lymph node and/or distant metastasis,the expressions of MMP-2 and MMP-9 were significantly higher in cases with lymph node and/or distant metastasis;（3）The expression of MMP-2 and MMP-9 in squmas cell carcinomas was significanty higher than of that in adenocarcinoma and adenosquamaous cell carcinoma（P〈0.05）;（4）The expression of MMP-2 and MMP-9 showed close correlation of each other（P〈0.05）.（5）The expression MMP-2 and MMP-9 showed close correlation with survival rate in patients with lung cancer（P〈0.05）.The 5-year survival rate in patients with high MMP-2,and MMP-9 expression was much lower than that in cases with low expression（P〈0.05）and（6）The results of multivariable Cox model analysis showed that detection of the expression of MMP-2 and MMP-9 might be helpful for predicting prognosis of patients with lung cancer.Conclusion：（1）Over-expression of MMP-2 and MMP-9 was very popular in lung cancer,and was closely related to the progression and metastasis of tumor and（2）Detection of the expression of MMP-2 and MMP-9 in lung cancer was helpful in predicting prognosis of lung cancer and guided the postoperative multimodality therapy for lung cancer.