糖尿病大鼠膀胱和脊髓背根神经节中P75的表达与尿流动力学改变

Expression of P75 in lumbar dorsal root ganglia and bladder of diabetic rats and urodynamic changes

目的探讨糖尿病大鼠膀胱与脊髓背根神经节(DRG)中神经生长因子受体P75的表达及尿流动力学改变。方法建立糖尿病大鼠模型20只,以15只正常大鼠为对照,应用免疫组化方法,分别检测大鼠膀胱组织及DRG中P75的变化情况,结合尿流动力学改变,探讨糖尿病膀胱病变(DC)可能的发病机制。结果与对照组比较,糖尿病组膀胱湿重增加[(171.150±6.081)vs.(135.530±5.330)mg],最大膀胱容量增加[(2.97±0.54)mLvs.(1.73±0.23)mL],储尿期膀胱压降低[(0.39±0.05)vs.(0.77±0.06)kPa],最大膀胱压力降低[(2.98±0.12)vs.(5.99±0.25)kPa](P均<0.05)。无论是在膀胱组织还是在DRG中,糖尿病组P75的表达均低于对照组,有统计学意义(P<0.05)。结论神经生长因子的受体P75在膀胱与脊髓背根神经节中的异常表达可能与DC的发病机制有关。

Objective To investigate the expression of P75 in lumbar dorsal root ganglia （DRG） and bladder of diabetic rats and the urodynamic changes. Methods 20 diabetes model of rats were built, with 15 normal rats as control. Expression of P75 in bladder and DRG was measured by immunohistochemical staining and urodynamic changes were measured. Results Compared with the control group., in the diabetic group, bladder wet weight increased [（171. 150±6. 081） vs. （135. 530±5.330）mg], the maximum bladder capacity increased E（2.97±0.54） vs. （1.73±0.23）mL], filling pressure of bladder decreased [（0.39±0.05） vs. （0.77± 0.06） kPa], and the maximum bladder pressure decreased [（2.98± 0.12） vs. （5.99±0.25）kPa] （P〈0.05）, expression of P75 decreased both in bladder wall （t =- 2. 589, P = 0. 010） and in DRG （t = - 2. 599, P= 0. 009）. Conclusion Reduction of P75 in bladder and DRG may be associated with the pathogenesis of diabetic cystopathy （DC）.