侵袭性牙周炎患者龈沟液中有机酸的研究

Analysis of short chain fatty acids in gingival crevicular fluid of patients with aggressive periodontitis

目的检测侵袭性牙周炎（aggressive periodontitis，AgP）患者龈沟液中7种有机酸的浓度，探讨其与AgP的关系。方法应用高效毛细管电泳技术，检测38例AgP患者152个位点和14名牙周健康对照者56个位点龈沟液中甲酸、琥珀酸、乙酸、乳酸、丙酸、丁酸和异戊酸的浓度，比较AgP组和健康对照组之间、AgP患者不同袋深组之间龈沟液中有机酸浓度的差别，分析出血指数（bleeding index，BI）、探诊深度（probing depth，PD）和附着丧失（attachment loss，AL）与有机酸浓度的相关关系。结果AgP组龈沟液中琥珀酸（1．00mmol／L）、乙酸（24．13mmol／L）、乳酸（8．33mmol／L）、丙酸（4．37mmol／L）、丁酸（2．33mmoL／L）和异戊酸（2．63mmol／L）的浓度均显著高于健康对照组（依次为0．33、11．35、3．88、2．07、0．00、0．00mmol／L，P〈0．05），甲酸浓度（7．08mmol／L）显著低于健康对照组（14．03mmol／L，P〈0．05）；除乳酸外其余6种有机酸在不同袋深组间浓度差异均有统计学意义（P〈0．05）；AgP组甲酸浓度与BI、PD和AL呈负相关，BI与琥珀酸、乙酸、乳酸、丙酸和丁酸浓度呈正相关，PD和AL与琥珀酸、乙酸、丙酸、丁酸和异戊酸浓度呈正相关。结论AgP的病变状况可能与龈沟液中琥珀酸、乙酸、丙酸、丁酸和异戊酸浓度升高而甲酸浓度降低有关。

Objective To investigate 7 short chain fatty acids （SCFA） concentrations in gingival crevicular fluid（GCF） of aggressive periodontitis （AgP） and to analyze the relationship between levels of SCFA and AgP clinical parameters. Methods GCF was collected from 152 sites of 38 AgP patients and 56 sites of 14 healthy subjects. Formic acid, suecinic acid, acetic acid, lactic acid, propionic acid, butyric acid and isovalerianic acid were detected by high performance capillary electrophoresis. Results The concentrations of succinic acid, acetic acid, lactic acid, propionic acid, butyric acid and isovalerianie acid in GCF were significantly higher in AgP patients than in healthy group, while formic acid was lower in GCF of AgP group compared with healthy group. Correlation analysis showed that formic acid was negtively correlated with bleeding index（BI） , probing depth（PD） and attachment loss（ AL）, while BI was positively correlated with succinic acid, acetic acid, lactic acid, propionic acid and butyric acid; PD and AL were positively correlated with suceinic acid, acetic acid, propionie acid, butyric acid and isovalerianic acid. Conclusions The elevation of succinic acid, acetic acid, propionic acid, butyric acid and isovalerianie acid concentrations in GCF may be related with AgP destruction condition, while formic acid concentration was reduced.