百合雄性生殖器官发育的分子生物学基础

Molecular Biology Mechanism of the Male Reproductive Organs Development in Lily

百合的雄性生殖器官花药和花粉较一般植物大,因此是研究植物生殖发育生物学的常用材料之一。近年来,许多研究者在百合花药、花粉和花粉管等雄性生殖器官和不同发育时期通过SDS-PAGE,蛋白质双向电泳,SSH和RT-PCR等手段分离到了部分特异表达的多肽和蛋白及cDNA片段;并且对部分多肽的功能进行了预测和验证。此外,还分离到一些编码百合在受精过程中花粉管与柱头互作的蛋白,并对其功能和可能的作用机制进行了推测。本文不仅介绍了百合雄性生殖器官发育相关的分子生物学机制,而且还对在相应研究过程中存在的一些问题及其在百合育种中的应用进行了探讨。

Male reproductive organs such as anther and pollen grain of lily （Lilium longiflorum L.） are generally much bigger than other plants＇. For this reason, it becomes one of the most used materials by researches for revealing the mechanisms underlying sexual reproductive development. In recent years, several specifically expressed peptides, proteins, and cDNA fragments had been isolated by researchers through SDS-PAGE, 2 D-protein electrophoresis, SSH, and RT-PCR technologies in pollen, anther, and pollen tube and different developmental stages in lily. Furthermore, function of part of these peptides had also been predicted and studied. Besides, some proteins had also been isolated that related with pollen tube and stigma interaction during fertilization. Their function had been inferred as well. In this review was not only issued on the molecular biology mecha- nism of the sexual reproductive development in lily, but also discussed the problems occur during these study and their possible application in lily breeding.