摘要
目的:观察乙肝病毒(hepatitis B virus,HBV)核酸疫苗pCMV-S2S免疫小鼠后,接种局部HBV preS2S抗原的表达及特异性细胞毒性T淋巴细胞(CTL)应答的动态变化。方法:将pCMV-S2S接种到BALB/c小鼠胫前肌,分别以免疫组化法检测接种局部肌肉HBV preS2S抗原表达的动态变化,乳酸脱氢酶(LDH)释放法检测特异性CTL活性的动态变化。结果:小鼠接种pCMV-S2S后,局部肌肉细胞3d后已有少量目的抗原HBV preS2S蛋白表达,4周时表达最强,至6月时仍可检出有preS2S蛋白表达,但表达强度明显减弱。小鼠接种pCMV-S2S3天后,特异性CTL活性平均值最高为21·67%,12周时CTL活性平均值最高为35·14%,6月时仍可检出较弱的CTL活性。结论:HBV核酸疫苗pCMV-S2S能在小鼠体内持续表达特异性抗原及诱生特异性CTL应答。
Objective:To observe the dynamic changes of specific antigen expression and cytotoxicity T lymphocyte (CTL) responses induced by a hepatitis B virus DNA vaccine pCMV-S2S in BALB/c mice.Methods:BALB/c mice were intramuscularly injected with pCMV-S2S.The expression of HBV specific antigen preS2S in the muscles were detected by immunohistochemistry assays.The specific CTL activities induced by mice were measured by lactate dehydrogenase (LDH) release assays.Results:HBV preS2S antigen expression in the mice muscles was detected 3 days after the injection of pCMV-S2S,increased to the peak 4 weeks after injection,and still could be detected 6 months later with weaker expression.Relative lower level of HBV preS2S-specific CTL lysis activities in pCMV-S2S mice was detected 3 days after injection.The average HBV preS2S-specific CTL lysis values achieved the peak by 35.14% 12 weeks after injection,and still kept at low level 6 months after injection.Conclusion:The hepatitis B virus DNA vaccine pCMV-S2S vaccination may elicit substantial HBV preS2S expression and HBV preS2S-specific CTL responses in vivo,and lasted for a relatively long time.
出处
《华西医学》
CAS
2008年第3期565-567,共3页
West China Medical Journal
基金
国家自然科学基金(No.30571640)
国家863高技术项目(No.2002AA214161)
四川省应用基础研究项目(No.04JY029-002-7)