精子形态与体外授精妊娠结局的关系

The relationship between sperm morphology and in vitro fertilization outcome

目的探讨精子形态与体外授精-胚胎移植(IVF-ET)妊娠结局的关系。方法对480对接受IVF-ET的男方精液进行分析,观察精子形态对IVF的受精率、卵裂率、优质胚胎率、胚胎冷冻率、着床率、妊娠率、流产率等的影响。结果随着精子正常形态率的降低,精子的质量逐渐下降,其密度、活动率,活动力都有显著下降(P<0.01);精子形态对卵子受精率及优质胚胎率有显著影响(P<0.01),但对辅助生殖中的其它参数,如卵裂率、胚胎冷冻率、着床率、妊娠率、流产率均无显著性影响(P>0.05)。结论精子形态在IVF中的作用主要是影响其受精率及胚胎的发育潜能,而对IVF妊娠的结局无预测作用。

Objective To investigate the relationship between sperm morphology and in vitro fertilization outcome. Methods Sperm morphology of 480 infertile husband＇s semen was analyzed by modified Papanicolaou staining. The effect of sperm morphology onfertilization, cleavage, good quality embryo, embryo cryopreservation, implantation, pregnancy and abortion rates were evaluated. Results Following with decrease of rate of normal morphology sperm, the concentration and motility of sperm were significantly decreased （P〈0.01）. IVF fertilization and good quality embryo rates in the group with high rate of normal morphology sperm（〉15%） were significantly higher than those in the other two groups with low rate normal morphology sperm（≤4% or 5%-15%） （P〈0.01）. There were no differences in the cleavage, embryo cryopreservation, implantation, pregnancy and abortion rates among the three groups （P〉0.05）. Conclusion Sperm morphology is involved in the IVF fertilization rate and embryo development potency. However, it could not be used topredict for IVF pregnant outcome.