对常规体外受精失败后补行卵胞浆内单精子注射的探讨

Rescue ICSI on oocytes failed to fertility after conventional IVF

目的探讨对可疑受精障碍的常规体外受精(IVF)周期在授精失败后补行卵胞浆内单精子注射(reacue ICSI)的时机及临床效果。方法对2006年1月至2007年6月在我中心接受常规IVF助孕治疗,可能有受精障碍的13例患者,取卵后6h授精,4h后观察,发现所有卵母细胞均未排出第二极体,立即行补救ICSI。结果13个周期共154个卵母细胞,其中MⅡ期142个;未行补救ICSI的46个均未受精;补救ICSI的96个,受精89个,受精率92.7%,移植13个周期29个胚胎,临床妊娠1例双胎和5例单胎,种植率24.1%。结论常规IVF受精后4h行补救ICSI可以获得较好的受精率和妊娠率。

Objectives To evaluate the feasibility and clinical effect of rescue ICSI on oocytes failed to fertility after convebtional IVF. Methods From January 2006 to June 2007, 13 patients were recruited in the study and received rescue ICSI. Conventional insemination was performed about 6 hours after oocytes retrieval. No second polar body was extracted 4 hours after insemination. Intracytoplasmic sperm injection were carried out immeditately in oocytes Results 154 oocytes were obtained from 13 patients. In 142 M Ⅱ oocytes, 46 oocytes with no rescue ICSI were all infertilized. A fertilization rate of 92.7% was achieved with rescue ICSI （89 of 96 oocytes）. 29 embryos transplanted in these 13 patients resulted in 6 pregnacies including 5 singleton and 1 twins, no abortion. Conclusion Applying rescue ICSI at 4 hours after insemination could improve fertilization rates and pregnancy rates