摘要
目的:探讨自体血清与来源于同一个体的组织培养之间的关系及比较兔自体血清与胎牛血清培养兔角膜缘细胞的不同。方法:采用组织块培养法,分别采用兔自体血清及胎牛血清培养兔角膜缘细胞。结果:兔血清培养72h,细胞从组织边缘游出,第8天,膜布满整个孔底,第10天细胞呈空泡样,第14天,膜化解;胎牛血清培养48小时,细胞从组织边缘游出,第8天,膜布满整个孔底,第11天细胞呈空泡样,第16天,膜化解。结论:从形态学上观察,兔自体血清和胎牛血清培养的兔角膜缘细胞无明显差别,兔自体血清可用于兔角膜缘细胞的培养。
Objective： To compare rabbit serum with fetal bovine serum method in the culture of rabbit limbal stem cells. Methods ： Cultured rabbit limbal stem cell with prepared rabbit serum and fetal bovine serum. Results ： With rabbit serum cultured, in 72h, cells emigrated from tissue edge, in 8 days, cells growthed like membrane, in 14 days, membrane dissolved. With fetal bovine serum cultured, in 48h, cells emigrated from tissue edge, in 8 days, cells growthed like membrane, in 16 days, membrane dissolved. Conclusion ： rabbit serum can use in rabbit limbal stem cell culture.
出处
《河南医学研究》
CAS
2008年第3期196-198,共3页
Henan Medical Research
