尾加压素Ⅱ对体外高糖培养大鼠肾小球系膜细胞周期的影响

Effect of Urotensin Ⅱ on Cycle of Rat Mesangial Cells Cultured in High Glucose Medium In Vitro

目的观察尾加压素Ⅱ(UⅡ)对体外高糖培养的大鼠肾小球系膜细胞周期的影响。方法体外高糖培养大鼠肾小球系膜细胞,加入不同浓度的UⅡ(10-7、10-8、10-9和10-10mol/L),37℃孵育24h,用碘化丙啶染色流式细胞术分析肾小球系膜细胞周期分布。结果UⅡ对体外高糖培养的大鼠肾小球系膜细胞有明显的促增殖作用,表现为细胞周期S期比例增加,以浓度为10-8mol/L时最明显。Ca2+阻断剂尼卡地平、Ca2+螯合剂EDTA及抗UⅡ抗体能明显抑制其促增殖作用。结论UⅡ能提高体外高糖培养的大鼠肾小球系膜细胞S期DNA合成速率,提示UⅡ对其具有明显的促增殖作用。

Objective To observe the effect of urotensin Ⅱ （U Ⅱ ） on the cycle of rat mesangial ceils cultured in high glucose medium in vitro. Methods Add the U Ⅱ at concentrations of 10^-7, 10^-8, 10^-9 and 10^-10 mol/L to the rat mesangial cells cultured in high glucose medium in vitro respectively. Incubate the mixtures at 37℃ for 24 h and determine the cycle of rat mesangial cells by flow cytometry with PI staining. Results U Ⅱ ,especially at a concentration of 104 mol / L, promoted the proliferation of rat mesangial cells cultured in high glucose medium in vitro significantly, expressed as the increased percentage of cells at S stage. The promoting effect was significantly inhibited by calcium ion blocker nicardipiue, calcium ion chalating agent EDTA and antibody against U Ⅱ . Conclusion U Ⅱ inereased the rate of DNA synthesis at S stage of rat mesangial cells cultured in high glucose medium in vitro, indicating the significantly promoting effect of U Ⅱ on proliferation of the cells.