不同程度猴脑缺血模型的CT灌注和脑血管造影评价

The DSA and CT Perfusion Evaluation of New Model of Artery Occlusion in Monkey

目的探讨不同程度脑缺血模型的建立以及栓塞剂量对猴自体血栓栓塞脑缺血模型的影响。材料与方法成年食蟹猴20只,随机分成5组:对照组、轻度缺血组、中度缺血组、重度缺血组和极重度缺血组,每组各4只。各组动物经介入法插管至大脑中动脉,对照组注入一定量的盐水,其他各组注入不同体积的自体血栓造成大脑中动脉血栓栓塞,介入血管造影(DSA)和CT灌注(CTP)扫描证明栓塞成功,分别于术后30min和120min行CTP扫描,测量脑血流平均通过时间(MTT)值,术后14天连续观察行为学表现。结果术后30minMTT:所有手术组MTT测量值明显高于对照组并且有显著差别;术后120minMTT:轻度缺血组和对照组未见明显差别,其他各组和对照组可见明显差别;术后死亡率:极重度组和重度组明显高于其他组,极重度组又明显高于重度组;术后行为学表现:重度组、中度组和轻度组可见明显行为学改变。各组有明显差异的数据均有统计学意义。结论猴不同程度脑缺血模型的建立,对研究脑缺血疾病有重要的作用;DSA和CTP对早期脑缺血疾病的诊断和预后判断有重要的价值。

Objective To investigate the role of DSA and CT perfusion （CTP） scanning in monkey middle cerebral artery occlusion （MCAo） model, which were established by various doses of fibrin embolus injected into the middle cerebral artery（MCA）. Materials and Methods 20 monkeys were randomly assigned to 5 groups including control, mild ischemia, moderate ischemia, serious and very serious ischemia groups. The various doses of embolus lodge were injected from the segment M1 of MCA in monkey and characterized their effects on cerebral blood flow by DSA and neurological deficit was observed in 14 days. CT perfusion was performed at 30 mins and 120 mins after operation to measure MTT values. Resuits The MTT values were significantly higher in operation groups than that in control group at 30 mins after operation, there was no significant difference between mild ischemia and control group, there was significant difference between moderate, serious, very serious and control group, MTT values in serious, very serious groups were higher than that in other groups, MTT values in very serious group were higher than that in serious group. Neurological deficit was observed in mild, moderate and serious ischemia groups. Conclusion Establishment of an embolic monkey model of stroke is important to study cerebral ischemia disease, DSA and CTP are helpful for early diagnosis and prognosis of cerebral ischemia disease.