原发性乳腺癌组织中KLK6蛋白和mRNA的表达及其临床病理学意义

Expression of KLK6 protein and mRNA in primary breast cancer and its clinical significance

目的:探讨KLK6蛋白及其mRNA在原发性乳腺癌组织中的表达和临床意义。方法:随机选取原发性乳腺癌患者88例并收集其术后标本,采用SABC免疫组化方法和RT-PCR技术,检测乳腺癌组织和正常乳腺组织中KLK6蛋白及其mRNA的表达。并分析其与原发性乳腺癌组织临床病理学特征之间的关系。结果:KLK6蛋白在原发性乳腺癌组织中的阳性表达率为78.40%(69/88),并与癌组织的临床病理学特征有关;发生淋巴结转移及ER(+)的癌组织中KLK6蛋白的阳性表达率明显低于未转移组(P<0.05)及ER(-)组(P<0.05)。KLK6 mRNA在原发性乳腺癌组织中的表达水平显著高于正常乳腺组织(P<0.01);但发生淋巴结转移的癌组织中,KLK6 mRNA的表达水平明显低于未转移组(P<0.05);ER+组亦低于ER(-)组(P<0.05)。KLK6蛋白及其mRNA的表达与乳腺癌相关基因CerbB-2的表达无相关性(P>0.05)。结论:KLK6蛋白及其mRNA的异常表达可能与原发性乳腺癌的发生、浸润、转移有关,可以作为一个肿瘤标志物在临床中应用。

AIM： To investigate the expression of KLK6 protein and mRNA in primary breast carcinoma, and to evaluate its clinical significance in clinicopathological status of patients with primary breast carcinoma. METHODS： 88 patients with primary breast carcinoma chosen randomly were examined for KLK6 protein and mRNA expression by SABC immunohistochemistry and RT-PCR, respectively. The association of KLK6 with the clinicopathologic features of the primary breast cancer was also analysis. RESULTS： The positive expression rates of KLK6 protein in breast carcinoma tissues were 78.40% （69/88）. Clinicopathological parameters of breast carcinoma were associated with KLK6 protein expression. KLK6 protein was expressed less frequently in breast carcinoma tissues with lymph nodes metastasis （P〈0.01） and ER＋ （P〈0.05）. Additionally, the mean expression level of KLK6 mRNA in cancerous tissues was significantly higher than that in normal tissues （P〈0.01）. However, KLK6 mRNA expression was negatively correlated with metastasis （P〈0.01） and ER status （P〈0.01）. The expression of KLK6 protein and mRNA in primary breast carcinoma tissues was not associated with that of CerbB-2. CONCLUSION： KLK6 expression in cancerous tissues may play an important role in the invasion and metastasis of primary breast carcinoma. The detection of it along with clinical staging may help to predict the prognosis of patients with primary breast carcinoma.