丹参注射液眼部电离子导入治疗玻璃体出血的实验研究

Treatment of danshen injection-iontophoresis on vitreous hemorrhage in rabbit

目的探讨丹参注射液电离子导入治疗玻璃体出血的疗效及机制。方法将兔实验性玻璃体出血模型18只(36只眼)分为对照组和治疗组,对照组不做任何治疗,观察出血的自然转归;治疗组予以丹参注射液眼部电离子导入治疗。分不同时间点抽取兔房水和玻璃体,用高效液相色谱法测定房水和玻璃体中的药物质量浓度。用检眼镜观察眼底玻璃体出血吸收情况。结果对兔实验性玻璃体出血,丹参注射液电离子导入组出血吸收较对照组快。结论丹参注射液电离子导入法可促进玻璃体出血的吸收,缩短病程,是一种简易而有效的眼局部治疗方法。

Objective This study was to investigate the effect and mechanism of danshen injection-iontophoresis on vitreous hemorrhage. Methods The vitreous hemorrhage model was induced by injecting into vitreous of 0.2 mL autohemorrhage in 36 eyes of 18 New Zealand white rabbits.2 mL of danshen solution was iontophorezed via ocular-cervical-occiput for 15 minutes per time and once per day for 20 times in dansen-treated group（9 rabbits）,and 9 model rabbits were as control group.Aqueous humor and vitreous were withdrawn at different time,and the drug concentration in aqueous humor and vitreous were detected with high-pressure liquid chromatography （HPLC）.The absorption status of vitreous hemorrhage was clinically evaluated by ophthalmoscope. Results There was no significant difference in dansen concentration between vitreous and aqueous humor in 0.5 hours and 2 hours（P〉0.05）,but significant difference was found in 5 hours（P〈0.01）.No obvious difference was seen in visuality of fundus before treatment between the two groups.In dansen-treated group,the absorption of vitreous hemorrhage was quicker than in the control group,and the number of visual fundus was remarkblely increased in comparison with control group,showing that the effect of danshen injection-iontophoresis was better than that in control group. Conclusion For vitreous hemorrhage,danshen injection-iontophoresis is an effective and simple approach to accelerating the absorption of vitreous hemorrhage and shorten the course of treatment.