SEL1L和p63蛋白在食管鳞状细胞癌及癌前病变中的表达

Expression and significance of SEL1L and p63 protein in esophageal squamous cell carcinoma and precarcinomacous lesion

目的研究SEL1L和p63蛋白在食管鳞状细胞癌和癌前病变中的表达。方法应用免疫组化EnVision二步法分别检测食管鳞状细胞癌(鳞癌组,n=60)、上皮内瘤变(高级别组n=32,低级别组n=13)和正常食管黏膜(对照组,n=33) SEL1L和p63蛋白的表达。结果鳞癌组及上皮内瘤变高级别和低级别组SEL1L蛋白的阳性表达率分别为96.7%、90.6%、61.5%,p63蛋白的阳性表达率分别为100.0%、100.0%、92.3%,与对照组SEL1L和p63蛋白的阳性表达率(6.1%和51.5%)比较差异均有统计学意义(P<0.01);Pearson相关分析显示,SEL1L和p63的表达与患者性别、年龄及肿瘤位置、大小、分化程度、浸润深度、淋巴结转移、临床分期均无明显相关性(P>0.05),但二者在高级别上皮内瘤变中的表达呈显著正相关(r=0.361,P<0.05)。结论SEL1L和p63在食管鳞癌演进过程中呈递增性过表达。提示二者可能参与食管鳞癌的发生机制并具有一定的协同作用。

Objective To investigate the expression of SEL1L and p63 protein in esophageal squamous cell carcinoma and precarcinomacous lesion. Methods Immunohistochemical staining （EnVision method ） was employed to detect the expression of SEL1L and p63 protein in 60 samples of esophageal squamous cell carcinoma, 32 samples of high grade esophageal intraepithelial neoplasia, 13 samples of low grade esophageal intraepithelial neoplasia and 33 samples of normal esophageal mucosa. Results The positive rate of SEL1L protein expression was 61. 5% in low grade intraepithelia neoplasia, 90.6% in high grade intraepithelial neoplasia and 96.7% in esophageal squamous cell carcinoma, significantly higher than that in normal esophageal mucosa （6.1%） （P 〈 0.01 ）. The positive rate of p63 protein expression was 92.3% in low grade intraepithelia neoplasia, 100.0% in high grade intraepithelial neoplasia and 100.0% in esophageal squamous cell carcinoma, also significantly higher than that in normal esophageal mucosa （51.5%） （ P 〈 0.01 ）. In addition, the positive correlation was found between the expression of SEL1L and p63 in high grade intraepithelial neoplasia（ r =0. 361, P 〈0. 05 ）. However, there was no significant correlation between the expression of SELI L and p63 protein and clinicopathologieal features such as age, gender, tumor location, tumor size, degree of tumor differentiation, depth of tumor invasion, lymph node metastasis and tumor clinical stage （ P 〉 0.05）. Conclusion Both the expression of SEL1L and p63 protein increases steadily in the progression of esophageal squamous cell carcinoma, which indicates that the two genes may play a role and cooperate with each other in the carcinogenesis.