c-kit蛋白和血小板源生长因子受体α在卵巢上皮性肿瘤中的表达

Expression of c-kit protein and platelet-derived growth factor receptor α in ovarian epithelial neoplasms

目的探讨c—kit蛋白、血小板源生长因子受体仪（PDGFRα）在卵巢上皮性肿瘤中的表达及其与卵巢癌临床病理的关系。方法采用免疫组织化学法（SP法）检测c—kit蛋白、PDGFRα，分析c—kit蛋白、PDGFRα在卵巢上皮性肿瘤中的表达与肿瘤分型、分化程度及国际妇产科联盟（FIGO）分期的关系。结果（1）c—kit蛋白在卵巢上皮性癌中表达的阳性率为50．70％，明显高于正常卵巢组织（10．00％）及良性卵巢肿瘤（20．00％）（P均〈0．01）。PDGFRtx在卵巢上皮性癌中表达的阳性率为63．38％，明显高于正常卵巢组织（15．00％）及良性卵巢肿瘤（25．00％）（P均〈0．叭）。（2）c—kit蛋白在浆液性卵巢癌中表达的阳性率高于黏液性卵巢癌（57．63％比16．67％，P〈0．05），而PDGFRa在浆液性及黏液性卵巢癌中表达阳性率差异无统计学意义（P〉0．05）。低分化卵巢癌组c—kit蛋白及PDGFRα表达的阳性率明显高于高中分化卵巢癌组（69．23％比40．00％，80．77％比53．33％，P〈0．05）。在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期卵巢癌中c—kit蛋白及PDGFRα表达的阳性率差异无统计学意义（P〉0．05）。（3）卵巢上皮性癌的c—kit蛋白和PDGFRα表达无相关性（P〉0．05）。结论c—kif蛋白与PDGFRα的表达与卵巢上皮性癌细胞的分化程度相关联：对c-kit蛋白与PDGFRa的检测可用于判断卵巢上皮性肿瘤的恶性程度及其预后。

Objective To investigate the expression of c-kit protein and platelet-derived growth factor receptor a（PDGFRa） in the ovarian epithelial neoplasms, and the relationships between their expression and clinical pathology. Methods c-kit protein and PDGFRα were detected by immunohistochemistry （SP method）. The relationships of their expression with the type, differentiation or FIGO clinical stage of the ovarian epithelial neoplasms were analyzed. Results （ 1 ） Intense c-kit protein immunostaining was observed in 50.70% of ovarian epithelial carcinoma, which was significantly higher than that of normal ovarian （ 10.00% ） and benign ovarian epithelial tumors （ 20.00% ） （ all P 〈 0. 01 ）. The positive expression rate of PDGFRα in ovarian epithelial carcinoma was 63.38% ,which was higher than that of normal ovarian （15.00%） and benign ovarian epithelial tumors （25.00%） （ all P 〈 0. 01 ）. （ 2 ） Compared with mucinous carcinoma, the expression of c-kit in the serous carcinoma was higher （57.63% vs 16.67% ,P 〈0. 05 ）. But the expression of PDGFRα has no difference between serous and mueinous carcinomas（ P 〉 0. 05 ）. In ovarian epithelial carcinoma, the positive staining of c-kit protein and PDGFRα in low differentiated grade group was significantly higher than that in high and middle differentiated grade group （69.23% vs 40. 00% ,80.77% vs 53.33% ,P 〈0. 05） . c-kit protein and PDGFRα expression had no difference between the FIGO clinical stages Ⅲ-Ⅳ and Ⅰ - Ⅱ （ P 〉 0.05）. （3）There was no significant correlation between the c-kit protein and PDGFRa expression （P 〉 0. 05 ）. Conclusions The expression of c-kit protein and PDGFRol is associated with histological grade of the ovarian epithelial neoplasm. The detection of c-kit protein and PDGFRcx may be helpful to estimate the malignant degree and the prognosis of the epithelial ovarian carcinoma.