七氟醚预处理对缺氧果蝇保护作用的全基因表达谱分析

Global Gene Expression Profiling of Protective Effects of Sevoflurane Preconditioning on Hypoxia Injury in Drosophila Melanogaster

目的:观察果蝇七氟醚预处理与缺氧后基因差异表达,探讨七氟醚预处理对缺氧损伤保护作用的分子生物学机制。方法:用CapitalBio的Drosophila Genome Oligo Set基因表达谱芯片对七氟醚预处理35 min与缺氧4 h果蝇的差异基因进行检测,与保护作用相关的基因我们用基因本体进行分析。结果:按差异显著阳性标准,从14593个基因中筛选出七氟醚预处理35min差异表达基因206条,其中170条表达上调,36条表达下调,缺氧4h差异表达基因66条,其中23条表达上调,43条表达下调,包括免疫诱导蛋白、信号转导、细胞外基质、细胞骨架蛋白、转录因子及核糖体蛋白等相关编码基因。结论:用cDNA表达谱芯片分析七氟醚预处理与缺氧基因表达,能快速筛选出七氟醚预处理保护作用相关基因,可以进一步探究七氟醚预处理保护作用的分子机制。

Objective：To observe differential gene expression in drosophila melanogaster with sevoflurane preconditioning and hypoxia and to study the molecular biological mechanisms of sevoflurane preconditioning against the anoxia injury.Methods：Changes in gene expression were studied by means of CapitalBio microarrays,at 35 min and 4 h after sevoflurane preconditioning/hypoxia.To identify functional pathways of interest,we used significantly regulated genes as input for gene ontology analysis.Results：According to the remarkable positive standard of the difference,206 genes which were differentially expressed at sevoflurane preconditioning 35 min were found from 14 593 candidate genes,and there were 170 genes upregulated,and 36 genes downregulated.Sixty-six genes which were differentially expressed at hypoxia 4 h were found from 14 593 candidate genes,and there were 23 genes upregulated,and 43 genes downregulated,including relevant code genes as immune induced protein,signal transduction,extacellular matrix,cytoskeletal protein,transcription factor,and ribosomal protein,etc.Conclusion：Gene expression profiles in drosophila melanogaster with sevoflurane preconditioning and hypoxia were analyzed using cDNA microarray,it can screen the genes related to protective effects of sevoflurane preconditioning quickly.This study provides further insight into the molecular mechanism of protective effects of sevoflurane preconditioning on hypoxia injary.