血小板自身抗体的流式细胞检测与意义

Flow Cytometry Detection of Platelet Associated Antibody in Patients with Idiopathic Thrombocytopenic Pupura

目的应用流式细胞仪(FCM)检测血小板抗体,研究其正常参考值及对特发性血小板减少性紫癜(ITP)诊断的价值。方法应用FCM检测20例正常人和10例ITP患者血小板表面抗血小板抗体,并与酶联免疫竞争抑制试验(ELISA法)进行比较。结果FCM检测20例正常人血小板表面IgG、IgA和IgM的荧光百分率分别为2.56±2.08%、13.75±5.98%and13.90±7.28%;ITP患者血小板表面IgG、IgA和IgM荧光百分率分别为28.13±17.74%、25.07±16.84%和23.22±17.85%。结论FCM对于检测血小板表面血小板相关抗体是快速、敏感的新方法,优于ELISA方法。

Objective To set up a method of detecting platelet associated antibody（PA Ig） by flow eytometry （FCM） ,and to investigate the value of FCM method in the diagnosis of idiopathic thromboeytopenic purpura （ ITP）. Methods We detected the platelet associated antibody of 20 healthy donors and 10 patients with ITP. These samples were also measured with ELISA. The results of FCM were compared with the results of ELISA. Results By FCM, the measured value of PA IgG, PA IgA and PA IgM, from 20 normal dornors, were 2. 56 ±2.08% ,13.75 ±5.98% and 13.90 ±7.28% , respectively. In patients with ITP, PA lgG, PA IgA and PA IgM were 28.13 ±17.74% ,25.07 ±16.84% and 23.22 ±17.85%. Conclusion FCM can be a rapid,sensitive and new method in detecting platelet autoantibody,and has better potential than ELISA.