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银杏叶提取物对有遗传过敏倾向脐血Th1/Th2免疫状态的影响 被引量:3

Effect of extract of Ginkgo Biloba leaf on Th1/Th2 cells of cord blood with hereditery allergic tendency
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摘要 目的:观察银杏叶提取物对有遗传过敏倾向新生儿脐血单个核细胞增殖和Th1/Th2失衡调节的影响.方法:选择20例新生儿父或母有过敏性疾病史(AD组)和20例新生儿父母均无过敏性疾病史(对照组)的正常足月新生儿脐血为研究对象.收集脐血血浆并分离单个核细胞(CBMCs),体外分别用银杏叶提取物联合植物血凝素(PHA)刺激培养24,48,72h,单独PHA刺激组设为对照.MTT比色法检测细胞增殖活性,酶联免疫吸附试验(ELISA)检测银杏叶提取物对细胞培养上清液中IL-2,IL-4,IFN-γ,GM-CSF的影响.结果:AD组IL-2,IL-4和GM-CSF高于对照组(P<0.05),IFN-γ无明显差异.银杏叶提取物干预培养48h后细胞因子中IL-2,IL-4,IFN-γ,GM-CSF均增多,且AD组与对照组相比差异有统计学意义(P<0.05).AD组中GB干预组与单纯PHA组比较IFN-γ/IL-4差异有统计学意义(P<0.01),IL-2/IL-4差异无统计学意义.AD组中GB干预后分析GM-CSF与IL-2和IFN-γ呈正相关关系(P<0.05)、与IL-4无相关性.结论:GB干预CBMCs后能调节Th1/Th2平衡,在上调Th1,Th2因子的同时,以上调Th1因子为主,从而调节过敏体质患儿的Th1/Th2失衡,为儿童变应性疾病的预防提供帮助. AIM: To investigate the effect of extract of Ginkgo Biloba leaf (GB) on cell proliferation of cord blood mononuclear cells(CBMCs) and the unbalance of Th1.Th2 cells with hereditary allergic tendency. METHODS: Forty normal full term neonates were divided into 2 groups [ allergic diseases (AD) group and control group] by their parents' anaphylactic diseases history. Blood plasma was collected and CBMCs were separated. After stimulating with PHA or PHA + GB respectively, the cells were cultured in vitro for 24,48,72 h. Cell proliferation was detected by MTT method. IL-2, IL4, IFN-γ, and GM-CSF in supernatant were assayed by ELISA. RESULTS : The expressions of IL-2, IL- 4, and GM-CSF in blood plasma were higher in AD group than in control group (P 〈 0.05 ), but IFN-3, was not different between the two groups ( P 〉 0.05 ). Expressions of IL-2, IL-d, IFN-γ, and GM-CSF were increased after GB stimulation for 48 h, with significant difference between AD group and control group (P 〈 0.05). In AD group, the expression ratio of IFN-γ/IL-4 in PHA + GB treatment cells was significantly higher than that in PHA treatment cells ( P 〈 0. 01 ) ; the expression ratio of IL-2/IL-4 was not different between PHA and PHA + GB treatment cells ; the expression of GM-CSF was positively correlated with the level of IL- 2 and IFN-γ/(P 〈0. 05 ) after GB stimulation, but not with level of IL-4 ( P 〉 0.05 ). CONCLUSION : GB can interfere the balance of Th1/Th2, especially up-regulating Th1, so it is useful to prevent the incidence of puerile allergic disease.
出处 《第四军医大学学报》 北大核心 2008年第21期1977-1979,共3页 Journal of the Fourth Military Medical University
基金 国家"十五"科技攻关项目(2004BA720A19-02)
关键词 银杏叶 脐血 淋巴细胞 TH1细胞 TH2细胞 Ginkgo Biloba leaf cord blood lymphocyte Th1 Th2
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