银杏叶提取物对有遗传过敏倾向脐血Th1/Th2免疫状态的影响

Effect of extract of Ginkgo Biloba leaf on Th1/Th2 cells of cord blood with hereditery allergic tendency

目的:观察银杏叶提取物对有遗传过敏倾向新生儿脐血单个核细胞增殖和Th1/Th2失衡调节的影响.方法:选择20例新生儿父或母有过敏性疾病史(AD组)和20例新生儿父母均无过敏性疾病史(对照组)的正常足月新生儿脐血为研究对象.收集脐血血浆并分离单个核细胞(CBMCs),体外分别用银杏叶提取物联合植物血凝素(PHA)刺激培养24,48,72h,单独PHA刺激组设为对照.MTT比色法检测细胞增殖活性,酶联免疫吸附试验(ELISA)检测银杏叶提取物对细胞培养上清液中IL-2,IL-4,IFN-γ,GM-CSF的影响.结果:AD组IL-2,IL-4和GM-CSF高于对照组(P<0.05),IFN-γ无明显差异.银杏叶提取物干预培养48h后细胞因子中IL-2,IL-4,IFN-γ,GM-CSF均增多,且AD组与对照组相比差异有统计学意义(P<0.05).AD组中GB干预组与单纯PHA组比较IFN-γ/IL-4差异有统计学意义(P<0.01),IL-2/IL-4差异无统计学意义.AD组中GB干预后分析GM-CSF与IL-2和IFN-γ呈正相关关系(P<0.05)、与IL-4无相关性.结论:GB干预CBMCs后能调节Th1/Th2平衡,在上调Th1,Th2因子的同时,以上调Th1因子为主,从而调节过敏体质患儿的Th1/Th2失衡,为儿童变应性疾病的预防提供帮助.

AIM： To investigate the effect of extract of Ginkgo Biloba leaf （GB） on cell proliferation of cord blood mononuclear cells（CBMCs） and the unbalance of Th1.Th2 cells with hereditary allergic tendency. METHODS： Forty normal full term neonates were divided into 2 groups [ allergic diseases （AD） group and control group] by their parents＇ anaphylactic diseases history. Blood plasma was collected and CBMCs were separated. After stimulating with PHA or PHA ＋ GB respectively, the cells were cultured in vitro for 24,48,72 h. Cell proliferation was detected by MTT method. IL-2, IL4, IFN-γ, and GM-CSF in supernatant were assayed by ELISA. RESULTS ： The expressions of IL-2, IL- 4, and GM-CSF in blood plasma were higher in AD group than in control group （P 〈 0.05 ）, but IFN-3, was not different between the two groups （ P 〉 0.05 ）. Expressions of IL-2, IL-d, IFN-γ, and GM-CSF were increased after GB stimulation for 48 h, with significant difference between AD group and control group （P 〈 0.05）. In AD group, the expression ratio of IFN-γ/IL-4 in PHA ＋ GB treatment cells was significantly higher than that in PHA treatment cells （ P 〈 0. 01 ） ; the expression ratio of IL-2/IL-4 was not different between PHA and PHA ＋ GB treatment cells ; the expression of GM-CSF was positively correlated with the level of IL- 2 and IFN-γ/（P 〈0. 05 ） after GB stimulation, but not with level of IL-4 （ P 〉 0.05 ）. CONCLUSION ： GB can interfere the balance of Th1/Th2, especially up-regulating Th1, so it is useful to prevent the incidence of puerile allergic disease.