急性坏死性胰腺炎肺损伤时RegⅠ基因表达

Overexpression of RegⅠ in lung injury of acute necrotizing pancreatitis

目的研究RegⅠ在急性坏死性胰腺炎(ANP)大鼠肺组织中的表达及其与ANP肺损伤的关系。方法健康成年SD大鼠随机分为假手术对照(C)组(30只)和ANP组(60只)。C组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射3%牛磺胆酸钠,制成ANP大鼠模型。观察胰腺和肺组织的病理改变及肺组织湿/干重比的改变,应用RT-PCR检测胰腺、肺组织中RegⅠmRNA的表达水平,并研究所观察指标与RegⅠ基因表达的相关性。结果与C组相比,ANP组肺组织中RegⅠ的mRNA水平过度表达。RegⅠ的表达水平分别与肺组织的病理学评分(r=0.5212,P<0.01)和肺组织湿/干重比(r=0.4797,P<0.01)呈正相关。结论ANP时大鼠肺组织中RegⅠmRNA的表达水平明显上调;RegⅠmRNA的表达与ANP所致的急性肺损伤的严重程度相关。

Objective To investigate the expression pattern of Reg Ⅰ in lung tissues of the rats with acute necrotizing pancreatitis （ANP）, and the correlation of Reg Ⅰ expression and lung injury. Methods SD rats were randomly allocated into the control group （n=30） and ANP group （n=60）. The rats in control group received laparotomy only. ANP model was established by injection of 3% sodium taurocholate into the pancreatic duct in ANP group. RT-PCR was performed to detect the Reg mRNA level in the pancreas and lungs. The pathologic changes of the pancreas and lung were observed. Results Reg Ⅰ mRNA was highly expressed in the lungs of ANP rats compared with that of control group. Reg Ⅰ expression level was positively correlated with lung pathological scores （r= 0.5212,P〈0.01） and lung tissue wet/dry ratio （r=0. 4797,P〈0.01）. Conclusion Reg Ⅰ is increased in lung tissues of ANP rats,and the expression level of Reg Ⅰ is correlated with the severity of lung injury.