茴三硫对染铝大鼠肝脏脂质过氧化作用及肝功能的影响

Effects of Anetholtrithione on Hepatic Lipid Peroxidation Induced by Aluminum in Rats

目的研究铝对大鼠肝功能及肝脏脂质过氧化的影响,并观察茴三硫对铝中毒大鼠的疗效。方法40只健康成年SD大鼠随机等分为4组,空白对照组用生理盐水腹腔注射及灌胃;染铝组腹腔注射AlCl3水溶液[6 mg/(kg.d)]及用生理盐水灌胃;茴三硫低、高剂量治疗组腹腔注射AlCl3水溶液[6 mg/(kg.d)],同时用茴三硫灌胃[10、20 mg/(kg.d)]。分别于第4和第8周末取血清检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和r-谷氨酰转移酶(GGT)、超氧化物歧化酶(SOD)、丙二醛(MDA)等项目,且在第8周末取肝组织测定SOD及MDA,并作肝组织病理检查。结果与空白对照组相比,染铝组大鼠的血清中ALT、AST升高,血清及肝组织中SOD活力减低、MDA水平升高,肝细胞变性、坏死及纤维组织增生;与染铝组比较,茴三硫治疗组血清中ALT、AST较低,血清及肝组织中SOD活力增高、MDA水平降低,肝脏病理学改变有所减轻。以茴三硫高剂量治疗组更为明显。结论铝可使大鼠肝功能下降,肝脏脂质过氧化水平升高及肝细胞受损,而茴三硫对铝的这种损害具有拮抗作用。

Objective To observe effects of aluminum on hepatic function and hepatic lipid peroxidation of rats and therapeutic effects of anethohrithione. Methods 40 adult and healthy SD rats were randomly divided into four groups. Normal saline was intraperitoneally injected and intragastrically administrated in blank group. AlCl3 water-solution [6 mg/（kg · d）] was intraperitoneally injected and normal saline was intragastrically administrated in chronic almninum exposed group. AlCl3 water-solution [6 mg/（kg · d）] was intraperitoneally injected and anethohrithione [-10, 20 mg/（kg ·d）] was respectively intragastrically administrated in low dose and high dose anethohrithione groups. Blood serum samples of all rats were collected in the 4th and the 8th week and ALT, AST, GGT, SOD, and MDA were detected. In addition, hepatic tissues were collected in the 8th week to measure SOD and MDA and to observe pathologic findings. Results Levels of ALT and AST in serum were higher, SOD activity was lower while MDA was higher in serum and hepatic tissue in chronically aluminum exposed group than those of the blank group. In addition, hepatic cell degeneration, necrosis and fibroplasias could be seen in chronic aluminum exposed group. Levels of ALT and AST were lower in serum, SOD activity was higher while MDA was lower in serum and hepatic tissue in anethohrithione groups than those of chronic aluminum exposed group. Hepatic pathological changes were improved in administration groups. More significant effects were shown in high anethohrithione dose group. Conclusions Aluminum could lead to lower the hepatic function, to elevate the hepatic lipid peroxidation, and to damage the hepatic cells. However, anethohrithione could antagonize these adverse effects.