肺成纤维细胞胶原表达的体外研究

Expression of the Collagen in Human Embryonic Lung Fibroblasts

目的探讨SiO2刺激矽肺患者的肺泡巨噬细胞(AM)是否通过影响人胚肺成纤维细胞(FB)Ⅰ型、Ⅲ型胶原合成,参与矽肺纤维化的发生发展。方法收集矽肺患者AM,体外经SiO2刺激18 h收集培养上清,与人胚肺FB共同孵育6、12、18、24、36、48、72 h,western blot方法分别检测FB培养上清中Ⅰ型、Ⅲ型胶原表达。结果经SiO2刺激矽肺患者AM条件上清,可促进FB培养液中Ⅰ型、Ⅲ型胶原的表达,各时间点Ⅰ和Ⅲ型胶原的比值无变化。结论SiO2通过AM影响了FB中Ⅰ型、Ⅲ型胶原表达,可参与肺纤维化的形成。

Objective To study the effect of cultured supernatant of human silicotic alveolar macrophages （AM） on the expression of the collagen type Ⅰ and collagen type Ⅲ in human embryonic lung fibroblasts. Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 18 h in vitro. The cultured supernatants were collected and cultured with human embryonic lung fibroblasts for 6, 12, 18, 24, 36, 48 and 72 h. Expression of collagen type Ⅰ and collagen type Ⅲ in fibroblast supernatants were detected with Western blot. Results The expression of collagen type Ⅰ and collagen type Ⅲ increased in cultured supernatant of silicotic AM exposed to SiO2. There was no obvious change in the value of collagen type Ⅰ/Ⅲ. Conclusions SiO2 may affect the expression of collagen type Ⅰ and type Ⅲ through AM intervention in the formation of lung fibrosis.