水飞蓟宾-磷脂酰胆碱复合物对氯仿所致肝损伤的保护作用

Protective Effect of Silybin-phosphatidylcholine Compound on Acute Liver Injury Induced by CHCl_3 in Mice

目的研究水飞蓟宾-磷脂酰胆碱复合物(SPC)对氯仿(CHCl3)所致小鼠肝损伤的保护作用。方法40只小鼠随机分成4组:正常对照组、CHCl3损伤组、CHCl3+SPC低剂量组、CHCl3+SPC高剂量组。前两组给予羧甲基纤维素钠(CMC-Na)混悬液灌胃,后两组分别给予SPC,连续8 d。最后一天给予SPC后2 h腹腔注射CHCl3致肝损伤,16 h后采血、分离血清,检测血清中ALT、AST活力,测肝组织中MDA和SOD值。结果损伤组小鼠血清中ALT、AST和肝组织中MDA值明显升高,SPC明显降低血清中ALT、AST值,SPC组肝组织中MDA值降低、SOD活力增加,SPC组与损伤组比较,差异有统计学意义(P<0.05)。结论SPC对CHCl3造成的急性肝损伤有明显的保护作用。

Objective To investigate the protective effect of silybin-phosphatidyleholine compound（SPC） on acute liver injury induced by CHCI3 in mice. Methods Forty mice were randomly and equally divided into four groups： normal group, liver injury group, SPC Ⅰ group and SPC Ⅱ group. Intragastric administration of 0.50/oo CMC-Na（sodium carboxymethycellulose）suspension were given to the normal and liver injury groups, and two dosages of SPC suspension（150 mg/kg and 300 mg/kg）were respectively given to SPC Ⅰ and SPC Ⅱ groups successively for eight days. Two hours after last administration of SPC, liver injury of animals was induced with 500 mg/kg of CHCI3. 16 h later, the levels of serum ALT, AST activity, and MDA and SOD in liver were detected by biochemical method. Results Administration of SPC greatly attenuated the lesion development by CHCl3, accompanied by great decrease in serum ALT, AST activity, lower hepatic content of MDA and increase activity of SOD（P〈0.05）. Conclusions SPC shows significant protective effect on CHCl3-induced hepatic injury in mice.