肝病患者Bactec 9120全自动血培养仪培养标本分析

Analysis on Cultured by Bactec 9120 Analysis on the Specimens of Patients with Hepatopathy by Bactec 9120 Cultuying Specimens of Patients with Hepatopathy

目的分析肝病患者Bactec 9120全自动血培养仪培养标本分离菌及阳性报警时间分布情况。方法培养3471瓶肝病患者标本,使用Phoenix100自动细菌鉴定仪进行细菌生化鉴定和药物敏感性分析。结果共分离出356株,42种病原菌,革兰阴性杆菌197株(55.3%,197/356),阳性球菌132株(37.1%,132/356),念珠菌24株(7.6%,24/356)。总阳性率10.3%,其中血培养阳性率11.2%,腹水培养阳性率4.3%,胆汁培养阳性率92.9%。分离率居前三位的病原菌依次为大肠埃希菌(20.2%),凝固酶阴性葡萄球菌(17.1%),肺炎及产酸克雷伯氏菌(12.9%)。在全部356例阳性瓶中,8h阳性报告率为17.1%,8h～24h阳性报告率为62.5%,24h～48h及72小时阳性率分别为14.0%、4.0%,>72h为2.4%。结论肝病患者的培养标本分离菌以革兰氏阴性杆菌为主,其中大肠埃希菌占首位,其次为凝固酶阴性葡萄球菌、肺炎及产酸克雷伯氏菌。培养标本在8h～24h阳性报告率最高。

Objective To analyze the distribution features of the bacteria cultured by Bactec 9120 from the specimens of patients with hepatopathy and positive alarm time. Methods Specimens from varies loci were cultured with Bactec 9120 blood cultural instrument. Bacteria were identified and drug sensitivity was tested by Phoenixl00 system. 3471 specimens were cultured, the positive rate being 10.3%, with 42 species and 356 strains obtained. Results The distribution of bacteria includes 197 Gram negative bacilli （55.3%）, 132 Gram positive cocci （37.1%） and 24 fungi （7.6%）. The positive percentage of blood culture is 11.2%, ascites 4.3%, and bile 92.9%. The most common pathogens are escherichia coli （20.2%）, coagulase negative Staphylococci （CNS） （17.1%）, Klebsiella pneumoniae and Klebsiella oxytoca（ 12.9% ）; In 356 positive bottles, the percentage of positive alarm time before 8 hours was 17.1%, 62.5% during （8～24）hours, 14.0% and 4.0% respectively during （24～48）hours and less than 72 hours and 2.4% over 72 hours. Conclusion The most common pathogens from specimens of patients with hepatopathy are Gram-negative bacilli. The positive alarm rate during（8～24） hours is higher than other time.