摘要
胶原酶两步灌流法获取SD大鼠的原代肝细胞,并用不同剂量的对乙酰氨基酚处理,MTT法测定肝细胞的存活率,分光光度法测定培养上清液中乳酸脱氢酶(LDH)的含量。Western blotting法测定大鼠肝细胞中细胞色素P450酶2E1(CYP2E1)的表达水平。结果表明,对乙酰氨基酚可导致肝细胞存活率降低,LDH含量显著升高,CYP2E1表达增加。说明达到一定的剂量时,对乙酰氨基酚可引起肝细胞的损伤。
Rat hepatocytes were obtained from SD rats by two step collagenase perfusion, and treated with different concentration of acetaminophen. The hepatocyte viability was measured by MTT, the activities of lactate dehydrogenase （LDH） was analyzed by spectrophotometer, and the expression of CYP 2E1 were measured by western blotting. With induction of acetaminophen, the hepatocyte viability decreased, the activity of LDH increased, the expression of CYP 2E1 increased. It demonstrated that acetaminophen could lead to hepatocyte injury when acetaminophen reached to higher dose.
出处
《中国农学通报》
CSCD
2008年第11期24-26,共3页
Chinese Agricultural Science Bulletin
基金
河北农业大学校长基金"中兽药药物代谢的分子机制研究"(2005-710)
