克伦特罗残留GC-MS检测方法的建立及对自制试纸条性能的确证

Development of GC-MS Method for Detecting Clenbuterol Residues and Confirmation of Self-made Strip Performance

建立了猪尿液中盐酸克伦特罗(CL)残留的GC-MS检测方法,并对自主研制的CL残留快速检测免疫金标试纸条(CL-Strip)进行了比较分析。结果表明,GC-MS的检测限为0.5ng/ml,CL-Strip的检测限为1.0ng/ml;CL-Strip与GC-MS两者的加标测定实验结果完全一致;用CL-Strip检测出的100份阴性尿样和18份阳性尿样,与GC-MS检测结果完全一致。说明CL-Strip与GC-MS同样灵敏、准确、稳定,由于CL-Strip具有简便、直观、快速、敏感、特异、准确等优点,建议在CL残留快速检测中推广应用。

GC-MS method of detection for Clenbuterol Hydrochloride （CL） residues in swine urine was established and compared with the method of self-made strip （CL-Strip）. The results showed that the detection limits of GC-MS and CL-Strip were 0.5ng/ml and 1.0ng/ml respectively. The detection results of CL added in samples were consistent completely. The detection results of 100 negative swine urine samples and 18 positive swine urine samples by CL-Strip method were agreed with GC/MS method analysis results. It can be concluded that the sensitivity, accuracy and stability of CL-Strip method were about at the same degree as GC-MS method, and CL-Strip method was recommended to be extended for its advantages in convenience, direct-viewing, rapidity, sensitivity, specificity and accuracy.