转反义trxs基因小麦种子萌发过程中碳代谢的变化

Change of Carbon Metabolism in Transgenic Anti-sense Thioredoxin s Gene Wheat in the Process of Germination

为了揭示反义硫氧还蛋白基因(anti-trxs)在抗穗发芽小麦中的作用机制;以转反义trxs基因小麦株系为材料,运用生理指标分析的方法,对转基因株系和对照种子萌发过程中硫氧还蛋白h活性、淀粉酶活性、可溶性糖和淀粉含量进行了检测;结果表明,转基因小麦籽粒trxh、α-淀粉酶和β-淀粉酶活性均显著低于对照;淀粉降解和可溶性糖生成速率明显下降。发芽1～5d,转基因小麦种子trxh、α-淀粉酶和β-淀粉酶活性分别比对照下降24.5%、40.4%和23.0%;淀粉降解和可溶性糖生成速率分别比对照降低23.7%和23%;说明,反义trxs基因对转基因小麦籽粒的碳代谢有调控作用。

To clarify the function mechanism of antisense - thioredoxin s （anti-trxs） gene in transgenic wheat with pre-harvest resistant sprouting characteristic, the thioredoxin h and amylase activities, starch and soluble sugar contents in transgenic line and non-transgenic cultivar ‘Yumai 34＇ were detected during seed germination stage. The results indicated that the activities of thioredoxin h, α- amylase and β-amylase were lowered significantly and the production velocities of starch degradation and soluble sugar decreased distinctly. During germinating days 1-5d, the activities of thioredoxin h, a-amylase and β-amylase were 24.5%, 40.4% and 23.1% lower than non-transgenic seeds, respectively. The velocities of starch degradation and soluble sugar producing decreased by 23.7% and 23%, respectively. The above result demonstrated that anti-sense trxs gene could regulate the carbon metabolism in the process of germination.