玉米丝黑穗病菌pra3基因的克隆及序列分析

Cloning and Sequence Analysis of pra3 Gene from Sporisorium reilianum

用PCR方法克隆了玉米丝黑穗病菌pra3基因,并对其序列进行了比较分析。结果表明:该基因大小为1 399 bp,具有3个内含子和4个外显子。比较发现,该基因片段与Schirawski等在GenBank上发表的序列同源性为100%。

pra3 gene of Sporisorium reilianum was cloned and sequenced. The result showed that the gene was 1399 bp, including three introns and four extrons. The homology with Schirawski＇s sequence （Accession No. ： AJ884590） was 100%.