摘要
为扩增蒙古绵羊Bcl-2基因3’端,根据GenBank上已公布的牛的序列,设计了2条引物。从蒙古绵羊脾中提取总RNA,采用RT-PCR技术扩增出Bcl-2的cDNA,并重组到PMD18-T载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的cDNA为Bcl-2,因为该cDNA包含由261个碱基组成的开放读码框(ORF),该ORF编码83个氨基酸。经比对,与牛的核苷酸序列的同源性为95.8%。
In order to amplify the end of 3＇of the Bcl-2 gene of Mongolia sheep , we designed two pair of primers according to the reported Bos taurus Bcl-2 gene sequence in the GenBank . Total RNA was extracted from the spleen of Mongolia sheep and the cDNA encoding Mongolia sheep bcl-2 was amplified by the reverse transcription PCR（RT-PCR）. The purified RT-PCR product was cloned in pBlueselect T vector. The results of restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing demonstrated that the Bcl-2 is belong to the family of Bcl-2 because the cDNA contain a open reading frame （ORF） of 261 bases which encoded 83 amino acid . The nucleotide sequences of Mongolia sheep Bcl-2 gene were compared with the counterpart sequences of Bos taurus , and the nucleotide homology was 95.8%.
出处
《中国畜牧兽医》
CAS
2008年第11期28-31,共4页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古自然科学基金资助项目(200202020402)
