增生性瘢痕增生过程中转化生长因子β表达的动态研究

Expression of transforming growth factor mRNA in hypertrophic scar

通过对３４例不同时期增生性瘢痕组织标本进行ＴＧＦβ－ｍＲＮＡ斑点杂交和原位杂交检测，结果发现：随着增生性瘢痕的发生、发展成熟，ＴＧＦβ－ｍＲＮＡ的总表达量明显呈由强至弱的变化，在１至６个月瘢痕组织内表达丰富，９个月时明显减弱，１２个月以后的瘢痕组织及正常皮肤对照标本中含量很少；ＴＧＦβ－ｍＲＮＡ表达的定位检测显示：除真皮层组织细胞有部分表达外，许多瘢痕表皮基底细胞内有较强表达。由此可见：①ＴＧＦβ表达与瘢痕增生发展有着密切联系；②ＴＧＦβ在瘢痕表皮组织中的表达对其自身增殖起抑制作用，导致表皮萎缩。

Trnsforming growth factor(TGF β) is an important growth factor with multifunctions in the processes of morphological development growth, regulation and wound healing.To realize the role of TGF β during the formation of hypertrophic scar(HS),we collected HS(34 cases)of different periods and analyzed expressions of TGF β mRNA in the tissues by dot blotting and hybridization in situ. The evidence was abtained that:① level of TGF β mRNA was higher in 1-6 month old HS than that in 9-12 month old HS,②expression of TGF β mRNA was located in epidermis as well as dermal in early HS. These results suggest that TGF β is a major factor in the overproliferation of fibroblasts and inhibiting proliferation of epidermal basal cells.