高效液相色谱法测定癸二酸和癸二腈

Determination of Sebacic Acid and Sebaconitrile by HPLC

采用C_(18)不锈钢柱为色谱柱(25 cm×4.6 mm,5μm),示差折光检测器,高效液相色谱法测定癸二酸和癸二腈。测定癸二酸时选用己二酸做内标物,甲醇和乙酸(1+99)溶液以体积比50比50混合的溶液作为流动相。在100～900 mg·L^(-1)范围内,峰面积比和峰高比对标准溶液的质量浓度呈线性关系。测定癸二腈时选用己二腈做内标,甲醇和乙酸(1+99)溶液以体积比40比60混合作为流动相。在0.227～8.17 g·L^(-1)范围内,峰面积比和峰高比对标准溶液的质量浓度呈线性关系。

A method of HPLC determination of sebacic acid and sebaconitrile using a stainless steel C18 column （25 cm × 4. 6 mm, 5 μm） and differential refractive index detector was proposed. Adipic acid and hexanedinitrile were selected as internal standards for determination of sebacic acid and sebaconitrile respectively. Eluants of mixture of methanol and dil. HOAc （1＋99）, mixed in the ratio of 50 to 50 （by volume） and in the ratio of 40 to 60 （by volume） were used as mobile phase in the elution of sebacic acid and sebaconitrile respectively. Linear relationships between values of peak area ratio （or peak height ratio） and concentration of the analytes were obtained in the range of 100-900 mg · L^-1 （for sebacic acid） and in the range of O. 227- 8. 17 g · L^-1 （for sebaconitrile）.