青岛地区婴幼儿诺如病毒分子生物学特点初步研究

A preliminary study on molecular of noroviruses detected in Qingdao

目的：了解青岛地区婴幼儿诺如病毒的分子生物学特点。方法：收集青岛市儿童医院2006年11～12月非细菌性腹泻大便样品48份，使用逆转录聚合酶链反应（RT—PCR）对粪便标本进行诺如病毒RNA检测，对RT—PCR阳性标本的PCR产物进行测序。结果：48例粪便标本中共有6例阳性，测序分析确定，3株为GⅡ／4型，1株为GⅡ／1型，1株的序列变化较大，但其和GeneBank里编号为EU072349的序列同源性较高为94％，一株由于检测不到信号而终止反应。结论：青岛地区婴幼儿病毒性腹泻中存在诺如病毒感染，且感染毒株的基因型别不一。

Objective： To investigate the molecular characteristics of noroviruses detected in Qingdao. Methods： A total of 48 samples were detected with noroviruses by a reverse transcription polymerase chain reaction （ RT - PCR） and the PCR products were sequenced, from feces of virus diarrhea in Qingdao children＇s hospital during Nov. to Dec. of 2006. Results： Among the 48 feces specimens, a total of 6 positive cases were identified. In the 6 noroviruses strains, 3 are GⅡ/4, 1 is GⅡ/1, 1 is variants and the homology with the strains EU072349 from GenBank is 94%, and the other cannot be sequenced. Condusion：Noroviruses are important etiological agents of viral gastroenteritis and there are more than two genotypes noroviruses in sporadic cases in children.