AccQ·Tag柱前衍生高效液相色谱法测定食品中添加的牛磺酸

Determination of additional taurine in foods by HPLC after derivatization

目的：建立AccQ·Tag柱前衍生高效液相色谱法测定食品中添加的牛磺酸的方法。方法：实验采用AQC衍生试剂，将样品进行柱前衍生后，经C18柱分离，荧光检测器检测。结果：被测组分在浓度为20～800μg／ml时，浓度与峰面积呈良好的线性关系（r=0．9999）；加标回收率为96．4％～112％；方法检出限0．5μg／ml；相对标准偏差（RSD）小于3％。结论：方法快速、简便、准确。

Objective： To establish a HPLC method for the determination of additional taurine in foods. Methods： After derivatization with AQC,taurine was separateded by Cls column and detected by FL detection. Results：The calibration curve was linear in the concentration range of 20 -800 μg/ml （r = 0. 9999）. The recoveries ranged from 96. 4% to 112%, detection limit 0. 5 μg/ ml and RSD less than 3 %. Conclusion：The method is fast,simple and accurate.