摘要
目的建立液质联用（HPLC—MS／MS）法同时测定人全血中环孢素A、他充莫司、西罗莫司的方法。方法采用Symmetry C18色谱柱（2．1mm×50mm，5μm），流动相：10mmol·l^-1醋酸铵-0．1％甲酸溶液和0．1％甲酸甲醇溶液为90：10，流速0．25mL·min^-1，采用多反应监测进行定量．结果环孢素A在人全血中50～1500μg·L^-1。内线性良好，他克莫司、西罗莫司在人全血中1～30μg·L^-1内线性良好，相对回收率在98．67％～103．56％之间，日内及日间精密度（PSD％）均小于10％。结论本方法能满足同时测定人全血中环孢素A、他克莫司、西罗莫司的要求，用于临床血药浓度的监测。
OBJECTIVE To develop a HPLC/MS/MS method for simuhanenus determination of cyelosporin A, tacrolimus and sirolimus in human blood. METHODS The separation was performed on a Symmetry C18 （2. 1 mm × 150 mm,5 μm） column with a mobile phase of water （containing 10 mmol· L^-1 ammonium acetate and 0. 1% formic acid） and methanol （containing 0. 1% formic acid）. Multi-reaction monitoring mode was employed for the quantitative determination. RESULTS The standard curve of cyclosporine was linear over the range of 50 - 1 500 μg · L^-1. The standard curves of tacrolimus and sirolimus were linear over the range of 1 - 30 μg · L^-1 , the relative recovel7 was among the range of 98.67% - 103.56% , RSDs of intraand inter-day validation were less than 10%. CONCLUSION HPLC-MS/MS can be nsed for the simultaneous determination of cyclosporin, tacrolimus and sirulimus in human blood.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2008年第21期1643-1647,共5页
Chinese Pharmaceutical Journal
