HPLC同时测定糖克软胶囊中5种木脂素类成分的含量

Simultaneous Determination of Five Lignan Compounds in Tangke Capsules by HPLC

目的建立反相高效液相色谱法同时测定糖克软胶囊中5种木脂素类(五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子甲素、五味子乙素)成分含量的方法。方法色谱条件:Agilent Extend C18柱;流动相为甲醇-2%四氢呋喃水溶液,线性梯度洗脱,流速0.8 mL·min-1;柱温50℃,检测波长254 nm。结果5种木脂素类化合物在实验浓度内线性关系良好,五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子甲素和五味子乙素的线性范围分别为15.8～1 575,5.76～576,4.65～465,3.61～361,10.6～1 057 mg·L-1,相关系数均大于0.999 0。平均加样回收率在100.2%～103.5%之间。5种木脂素类化合物含量重复性试验RSD小于1.98%。结论本方法简单、灵敏、重复性好,可用于糖克软胶囊质量控制。

OBJECTIVE To develop a high performance liquid chromatography method for the simultaneous determination of five major lignans （schisandrin, gomisin A, angeloylgomisin H, deoxyschizandrin and γ-schisandrin） in Tangke capsule. METHODS Chromatographic separation was performed on an Agilent Extend C18 column. The mobile phase was composed of methanol and 2% tetrahydrofuran-water at a linear gradient elution. The column temperature was maintained at 50 ℃. Detection wavelength was 254 nm, and flow rate was 0. 8 mL · min ^-1. RESULTS Good linear relationships of five lignans were provided over investigated concentration ranges. The linear ranges of schisandrin, gomisin A, angeloylgomisin H, deoxyschizandrin and 3,-schisandrin were 15.8 - 1 575, 5.76-576, 4. 65-465, 3.61 -361, 10. 6 -1 057 mg · L^-1, respectively. The values of correlation coefficient were higher than 0. 999 for all the analytes. The average recoveries of the five components ranged from 100. 2% to 103.5%. Repeatability experiments showed that relative standard deviation （RSD） values of their contents were less than 1.98%. CONCLUSION The developed method is simple, sensitive and repeatable. The method can be used for quality control of Tangke capsule.