紫外激光诱导组织自体荧光诊断肺癌和肝癌

Preliminary Studies of UV Laser Induced Autofluorescence of Tissues in the Diagnosis of Lung and Liver Cancer

测量了３０８ｎｍ紫外激光诱导肺正常和癌组织各７例、肝正常和癌组织各６例在３１０ｎｍ至５５０ｎｍ波段的自体荧光，目的是根据光谱建立一种将正常组织与癌组织区别开来的方法。结果提示：癌组织与正常组织荧光光谱的主要区别是４６０ｎｍ附近次峰强度不同，从而导致了３４０ｎｍ处荧光强度与４４０ｎｍ处荧光强度比（Ｉ３４０／Ｉ４４０）不同。肺正常和癌组织平均Ｉ３４０／Ｉ４４０值分别为１．３０４±０．１９５和２．１２４±０．４１２（Ｐ＜０．０１）；肝正常和癌组织平均Ｉ３４０／Ｉ４４０值分别为２．３６３±０．６７０和１．６７５±０．２２０（Ｐ＜０．０５）。根据癌组织与正常组织Ｉ３４０／Ｉ４４０值差异，可把癌组织与正常组织区分出来，为早期诊断恶性肿瘤提供了一种新的方法。

In order to establish a way to distinguish cancerous from normal tissues by autofluorescence spectroscopy, the autofluorescence of 7 normal and 7 cancerous tissues of the lung, 6 normal and 6 cancerous tissues of the liver were measured. The range of fluorescence spectra is from 310 nm to 550 nm. The results showed that the primarily difference between normal and cancerous tissues is the intensity difference of the second peak around 460 nm. It leads to the difference of the radio I 340 /I 440 . The mean value of the ratio was 1 304±0 195 for normal and 2 124±0 412 for cancerous lung tissues (P<0 01), 2 363±0 670 for normal liver and 1 675±0 220 for cancerous tissues of the liver (P<0 05). Based on those differences, cancerous tissues can be distinguished from normal tissues. Therefore, a new method for diagnosing malignant neoplasia might be developed.