膀胱癌和肾癌中RASSF1A和BLU的甲基化状态

Methylation of RASSF1A and BLU in bladder carcinoma and renal carcinoma

目的探讨RASSF1A和BLU在膀胱癌和肾癌中启动子甲基化状态及其意义。方法采用甲基化特异性PCR(MSP)技术分别在45例膀胱癌组织和相应癌周正常组织、12例肾癌组织及其相应癌周正常组织和3例非肿瘤患者正常膀胱组织中检测RASSF1A和BLU启动子区域的甲基化状态。结果膀胱癌组织中,RASSF1A和BLU甲基化率分别为55.6%和31.5%;肾癌组织中,两基因甲基化率为66.7%和33.3%。RASSF1A和BLU启动子甲基化状态与膀胱癌临床病理参数无明显相关性(P>0.05)。在膀胱癌或肾癌中,RASSF1A和BLU启动子甲基化状态之间均无明显相关性(P>0.05)。结论膀胱癌和肾癌中,RASSF1A和BLU频繁发生高甲基化,高甲基化可能参与膀胱癌和肾癌两类肿瘤的发生。

Objectives To investigate the promoter methylation status of RASSF1A and BLU in bladder carcinoma and renal carcinoma and their significance.Methods The methylation status of promoter of RASSF1A and BLU were examined by MSP in 54 cases of bladder carcinoma tissues and adjacent normal tissues from 45 cases,12 cases of renal carcinoma tissues and adjacent normal tissues and 3 cases of normal bladder tissues from noncancerous patients.Results Hypermethylation of RASSF1A and BLU was detected in 55.6% and 31.5% of bladder carcinoma tissues,retrospectively.In renal carcinoma tissues,hypermethylation of RASSF1A and BLU was detected in 66.7% and 33.3%,respectively.No association between the methylation status of RASSF1A and BLU with the clinical pathological parameters of bladder carcinoma was found（P〉0.05）,neither was it found between the methylation status of RASSF1A and BLU in bladder carcinoma or renal carcinoma（P〉0.05）.Conclusions RASSF1A and BLU are found with frequent hypermethylation in bladder carcinoma and renal carcinoma,which may be involved in tumorigenesis.