血脂康胶囊的体外抗肿瘤作用

Anti-neoplastic Effect of Xuezhikang in vitro

目的研究血脂康胶囊的体外抗肿瘤作用及其对化疗药物氟尿嘧啶(5-Fu)抗肿瘤作用的影响。方法采用四甲基偶氮唑盐(MTT)法对血脂康单用及其与5-Fu合用后对人肝癌细胞HepG2、乳腺癌细胞MCF-7和白血病细胞HL-60的抑制率进行检测。结果血脂康(2.56mg/ml、0.64mg/ml、0.16mg/ml、0.04mg/ml、0.01mg/ml)对HepG2、MCF-7和HL-60肿瘤细胞的抑制作用呈剂量依赖性,其IC50分别为14.057mg/ml、19.859mg/ml和27.771mg/ml;不同浓度的血脂康(0.64mg/ml、0.16mg/ml、0.04mg/ml、0.01mg/ml、0.0025mg/ml)合并5-Fu(0.24mg/ml)对3种人癌细胞的抑制率均显著高于单用5-Fu(0.24mg/ml)。结论血脂康对体外培养的癌细胞具有显著抑制作用,并可显著增强化疗药物5-Fu的抗肿瘤作用。

Objective To study the anti-neoplastic effect of Xuezhikang （Regulate blood-fat） Capsule in vitro and its influence on the chemotherapeutic drug 5 Fluorouracil （5-Fu）. Methods Using MTT method to measure the inhibition rate of Xuezhikang Capsule used simply, 5-Fu used simply and Xuezhikang Capsule with 5-Fu together on HepG2, MCF-7 and HL-60 cells. Results The inhibitory action of Xuezhikang Capsule（2.56, 0. 64, 0. 16, 0. 04, 0.01 mg/ml） on HepG2, MCF-7 and HL-60 cells was dose-depen- dent and the IC50 was 14. 057, 19. 859 and 27. 771mg/ml respectively. The inhibition ratio of five different doses of Xuezhikang Capsule（0.64, 0.16, 0. 04, 0. 01, 0. 0025 mg/ml） with 5-Fu （0. 24mg/ml） together all remarkably higher than that of 5-Fu used simply. Conclusion Xuezhikang Capsule has a significant anti-cancer effect in vitro and can enhance remarkably the anti-neoplastic effect of 5- Fu.