摘要
综述了离体培养、有利基因的克隆及遗传转化等生物技术的三个方面在荔枝研究中的应用进展。已有研究者分别建立了花粉培养、叶片培养、幼胚培养等获得植株的再生体系,克隆了部分荔枝基因,建立了基因枪与根癌农杆菌转化体系。这些研究为荔枝生物技术育种提供了基础。
The advances in research on in vitro culture, gene cloning and genetic transformation of litchi were reviewed in this paper. Plant regeneration from pollen, leaves and young embryo had already been succeeded. Some useful genes had been cloned and the genetic transformation mediated by particle bombardment and Agrobacterium turnefaciens infection had been constructed. The problems and prospects of litchi biotechnology were also discussed.
出处
《广西植物》
CAS
CSCD
北大核心
2008年第6期832-836,734,共6页
Guihaia
基金
国家自然科学基金(30460085)
海南省教育基金(Hj200534)
中央级公益性科研院所基本业务费专项(ITBBZD2007-3-1)~~
关键词
荔枝
生物技术
离体培养
基因克隆
遗传转化
litchi
biotechnology
in vitro culture
gene cloning
genetic transformation
